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Öğe Multilocus sequence analysis reveals the genetic diversity of European fruit tree phytoplasmas and supports the existence of inter-species recombination(Microbiology Soc, 2011) Danet, Jean Luc; Balakishiyeva, Gulnara; Cimerman, Agnes; Sauvion, Nicolas; Marie-Jeanne, Veronique; Labonne, Gerard; Lavina, AmparoThe genetic diversity of three temperate fruit tree phytoplasmas 'Candidatus Phytoplasma prunorum', 'Ca. P. mali' and 'Ca. P. pyri' has been established by multilocus sequence analysis. Among the four genetic loci used, the genes imp and aceF distinguished 30 and 24 genotypes, respectively, and showed the highest variability. Percentage of substitution for imp ranged from 50 to 68% according to species. Percentage of substitution varied between 9 and 12% for aceF, whereas it was between 5 and 6% for pnp and secY. In the case of 'Ca P. prunorum' the three most prevalent aceF genotypes were detected in both plants and insect vectors, confirming that the prevalent isolates are propagated by insects. The four isolates known to be hypo-virulent had the same aceF sequence, indicating a possible monophyletic origin. Haplotype network reconstructed by eBURST revealed that among the 34 haplotypes of 'Ca. P. prunorum', the four hypo-virulent isolates also grouped together in the same clade. Genotyping of some Spanish and Azerbaijanese 'Ca. P. pyri' isolates showed that they shared some alleles with 'Ca. P. prunorum', supporting for the first time to our knowledge, the existence of inter-species recombination between these two species.Öğe POTENTIAL PSYLLID VECTORS OF CANDIDATUS PHYTOPLASMA MALI AND CANDIDATUS PHYTOPLASMA PYRI IN TURKEY(Univ Agriculture, Fac Veterinary Science, 2016) Kaya, Kamuran; Serce, Cigdem Ulubas; Gazel, Mona; Caglayan, Kadriye; Sauvion, NicolasPsyllids are vectors of phytoplasma, which cause important diseases of pome fruit trees. Psyllid species reported as phytoplasma vectors were captured during 2010-2011 in several pome fruit growing regions in Turkey. Potential psyllid vectors of 'Candidatus Phytoplasma mali' were collected from Malus spp. (apple), Cydonia oblonga (quince), Crataegus spp. (hawthorn) and also from the overwintering hosts, whereas those of 'Candidatus Phytoplasma pyri' were collected from wild and cultured forms of Pyrus spp. (pear) trees. The psyllids were identified morphologically as Cacopsylla picta, C. melanoneura-C. affinis complex, C. crataegi, C. pyrisuga, C. pyri, C. pyricola and other Cacopsylla species. The highest natural phytoplasma infection rate was found in C. picta followed by C. pyri, C. melanoneura-C. affinis complex and C. crataegi with rates of 4.36, 3.84, 2.77 and 1.67%, respectively. No phytoplasma were detected in C. pyrisuga, C. pyricola, or the other Cacopsylla spp. 'Ca. P. mali' was detected in C. picta, C. melanoneura-C. affinis complex and C. pyri; 'Ca. P. pyri' was detected in C. picta, C. crataegi, C. melanoneura-C. affinis complex and C. pyri individuals. To our knowledge, this is the first report on the possible psyllid vectors of 'Ca. P. mali' in Turkey.Öğe Survey on the presence of Cacopsylla pruni in Turkey: preliminary results(Alma Mater Studiorum, Univ Bologna, 2011) Serce, Cigdem Ulubas; Yvon, Michel; Kaya, Kamuran; Gazel, Mona; Cengiz, Feza Can; Caglayan, Kadriye; Sauvion, NicolasAlthough 'Candidatus Phytoplasma prunorum' has been previously detected in different regions of Turkey, its vectors have not been identified yet. A survey was conducted in 2010 and 2011 in order to determine the presence of Cacopsylla pruni populations in six different fruit tree producing areas. The psyllid was found in Adana, Mersin, Bursa and Yalova on either Prunus spinosa or wild plums (Prunus spp.) and also on fir trees. The mean percentage of individuals of C. pruni infected with Ca. P. prunorum was around 23% in Mersin.