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Öğe Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm(Israeli Journal Of Aquaculture-Bamidgeh, 2005) Bozkurt, Y; Akcay, E; Tekin, N; Secer, SRainbow trout (Oncorhynchus mykiss) sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in their own extenders at 30 degrees C for 30 s. For both freezing methods, the best fertilization results were obtained with extender III containing 15% DMSO, 52.3% (56.3% of the control) for sperm frozen in straws and 48.4% (52.4% of the control) for sperm frozen as pellets. The interaction between extender and cryoprotectant was statistically significant (p<0.05).Öğe Effect of short-term preservation of mirror carp (Cyprinus carpio) semen on motility, fertilization, and hatching rates(Israeli Journal Of Aquaculture-Bamidgeh, 2005) Bozkurt, Y; Secer, SMirror carp (Cyprinus carpio) semen was collected by hand stripping for short-term preservation. Two groups of pooled semen were diluted with one of two extenders; a control group was undiluted. The three groups were stored at 4 degrees C for 72 h. During preservation, spermatozoa motility (%) and duration of spermatozoa movement (s) were determined every 12 h. Following preservation, fertilization was carried out using the dry fertilization technique at 1 x 10(5) spermato-zoa/egg. The highest fertilization rates were 21 +/- 3.60% in May and 16.67 +/- 2.08% in June, both significantly higher than in the control. The highest hatching rates, 69.60 +/- 11.86% in May and 89.13 +/- 10.75% in June, did not significantly differ from the control.
