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Öğe CHANGES IN CHEMICAL COMPOSITION, ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC CONTENT OF ARBUTUS ANDRACHNE FRUIT AT DIFFERENT MATURATION STAGES(Chiriotti Editori, 2009) Ozgen, M.; Torun, A. A.; Ercisli, S.; Serce, S.Several characteristics of Arbutus andrachne L. fruit harvested at four maturation stages (green, green-orange, orange-red and red) have been examined. Soluble solids, pH, acidity, organic acids, specific sugars, and several nutrient element concentrations (N, P, K, Ca, Mg, Fe, Zn, Mn, Cu) have been determined. The total phenolic content along with antioxidant activity, determined by FRAP and TEAC methods, were also measured. There were significant differences among most of the traits tested. The average soluble solids increased from 16.0 to 20.6% going from the green to the red stages, while the acidity decreased from 0.54 to 0.45%. All of the element concentrations were statistically different among the various stages, with N and Ca being the most variable. The total acidity was found to be the highest in the orange-red and red stages (0.52 and 0.50 g/100 g, respectively). The main organic acid was malic acid (0.32 g/100 g) followed by ascorbic and citric acids (0.09 and 0.03 g/100 g) respectively. The red (17.84 g/100 g) and orange-red (16.25 g/100 g) stages had the highest total sugar values. Fructose was the main sugar in the fruit with an overall average of 8.91 g/100 g. The total phenolic content was highest at the red stage (3,904 mg gallic acid (GA)/kg fresh weight (fw)), followed by the green stage (3,589 mg GA/kg fw). The antioxidant activities were highest at the red stage as determined by both the TEAC and FRAP methods (26.5 and 21.8 mu mol TE/g fw).Öğe Changes in chemical composition, antioxidant activities and total phenolic content of Arbutus Andrachne fruit at different maturation stages(Codon Publications, 2009) Özgen, M.; Torun, A.A.; Ercisli, S.; Serce, S.Several characteristics of Arbutus andrachne L. fruit harvested at four maturation stages (green, green-orange, orange-red and red) have been examined. Soluble solids, pH, acidity, organic acids, specific sugars, and several nutrient element concentrations (N, P, K, Ca, Mg, Fe, Zn, Mn, Cu) have been determined. The total phenolic content along with antioxidant activity, determined by FRAP and TEAC methods, were also measured. There were significant differences among most of the traits tested. The average soluble solids increased from 16.0 to 20.6% going from the green to the red stages, while the acidity decreased from 0.54 to 0.45%. All of the element concentrations were statistically different among the various stages, with N and Ca being the most variable. The total acidity was found to be the highest in the orange-red and red stages (0.52 and 0.50 g/100 g, respectively). The main organic acid was malic acid (0.32 g/ 100 g) followed by ascorbic and citric acids (0.09 and 0.03 g/ 100 g) respectively. The red (17.84 g/ 100 g) and orange-red (16.25 g/100 g) stages had the highest total sugar values. Fructose was the main sugar in the fruit with an overall average of 8.91 g/ 100g. The total phenolic content was highest at the red stage (3,904 mg gallic acid (GA) /kg fresh weight (fw)), followed by the green stage (3,589 mg GA/kg fw). The antioxidant activities were highest at the red stage as determined by both the TEAC and FRAP methods (26.5 and 21.8 LImol TE/g fw).Öğe Collection and morphological characterization of Lagenaria siceraria germplasm from the Mediterranean region of Turkey(Springer, 2008) Yetisir, H.; Sakar, M.; Serce, S.The landraces of Lagenaria siceraria in Turkey show great diversity for morphologic traits, particularly in fruit size and shape even though Turkey is not centre of genetic diversity for L. siceraria. In Turkey, L. siceraria is used as food, musical instruments and containers, according to the type and shape of their fruits. Its diversity has been gradually declining over the last 25 years. With the aim of assessing variations in plant, fruit and seed morphology among the L. siceraria landraces, 15 field trips for collection of germplasm to southern parts of Turkey (Mediterranean region) were carried out in the period of 2003-2004. The study also aimed at developing a representative core collection of the material to guide future studies and uses concerning its existing genetic diversity in Turkey. A total of 182 accessions (fruits and/or seeds) were collected. The seeds from all the sample material were sown in green house conditions at the experimental station of the Faculty of Agriculture of the Mustafa Kemal University in Hatay (Antakya), (36A degrees 18' 22'' N, 36A degrees 13' 33'' E, 82 m). In the spring of 2004, 162 out of the 182 sown seeds germinated and developed into viable seedlings. These were further transplanted in the field in 10 replicates. The morpho-agronomic characterization was carried out following the international standards for crop descriptors set by Bioversity International. The data were subject to both descriptive statistics and multivariate analysis by Principle Component Analysis. The descriptive statistics revealed that the whole collection exhibits a great deal of morphological diversity and that the subset core collection represents most of the variability. The first three principle components, calculated by using six quantitative traits, explained 26%, 21% and 17% (a total of 64%) of the total variation. Among the studied accessions, no apparently distinct patterns such as geographical origin were detected. This may suggest that the accessions have been introduced to Turkey from multiple locations and/or their diversity had been distributed almost evenly across the Mediterranean region of this country. Based on our results from the morphological characterization, 30 genotypes were selected to develop a subgroup (core) collection in order to represent most of the genetic diversity of all accessions. The work presented here is the first report about morphological characterization of Turkish L. siceraria germplasm.Öğe The Current and Future Situations for Sweet Cherry Production in the Eastern Mediterranean Region, Hatay, Turkey(Int Soc Horticultural Science, 2008) Polat, A. A.; Durgac, C.; Serce, S.In most countries, large-scale sweet cherry (Prunus avium L.) production usually occurs in small climatically suitable regions. There are large areas in Turkey that are suitable for sweet cherry production, such as Hatay, which is located in the eastern part of the Mediterranean region with production centered in Belen and Iskenderun. According to 2004 data, 530 t of sweet cherries were produced in Hatay. Of this, 59% was from Belen and 36% from Iskenderun. Studies to determine production potential and possible problems in the Hatay region revealed that the origins of the sweet cherries grown in the region are unknown, and an important cultivar mixture was observed as well. Moreover, significant problems were determined for orchard establishment, cultivar selection, irrigation, fertilization, pruning, cultivation techniques, harvesting and postharvest applications. Future studies to find solutions to these problems should increase sweet cherry production and may, therefore, increase both domestic consumption and export potential.Öğe Gelling agents and culture vessels affect in vitro multiplication of banana plantlets(Funpec-Editora, 2010) Kacar, Y. A.; Bicen, B.; Varol, I.; Mendi, Y. Y.; Serce, S.; Cetiner, S.Agar is the most commonly used gelling agent in media for plant tissue culture. Because of the high price of tissue-culture-grade agar, attempts have been made to identify suitable alternatives. The type of culture vessel and lid also affects the gaseous composition inside the vessel as well as light penetration. In turn, the vessel affects growth parameters, such as shoot elongation, proliferation and fresh weight, as well as hyper-hydric degradation processes. We examined the effects of different culture vessels, including commercial glass jars, magenta boxes, and disposable containers, as well as different gelling agents (agar-agar, Agargel, Phytagel, and plant agar) on the micropropagation of Dwarf Cavendish bananas in an effort to find a combination that yields large numbers of high-quality seedlings. The different culture vessels did not significantly affect seedling culture success. The medium significantly affected shoot weight. Phytagel resulted in the highest shoot weight (overall mean = 2.4 g), while agar, Agargel and plant agar resulted in 1.7, 2.2 and 2.2 g, respectively. Disposable container/Phytagel and Magenta/Agargel combinations yielded the highest shoot weights (2.9 and 3.0 g, respectively). Mean shoot length increased progressively with subculture (four subcultures were made). The highest mean shoot length was obtained with Phytagel and Agargel media (6.4 and 6.3 cm, respectively). Shoot number was significantly affected by medium only at subculture 4. Overall, the highest mean shoot length was obtained with the Magenta/Agargel combination (8.5 cm). Phytagel and plant agar gave higher mean shoot number than agar and Agargel (2.1, 2.1 and 1.7 and 1.9, respectively). The costs of the media and of the culture vessels need to be taken into account for final choice of the banana shoot culture system.Öğe Karyological features of wild and cultivated forms of myrtle (Myrtus communis, Myrtaceae)(Funpec-Editora, 2010) Serce, S.; Ekbic, E.; Suda, J.; Gunduz, K.; Kiyga, Y.Myrtle is an evergreen shrub or small tree widespread throughout the Mediterranean region. In Turkey, both cultivated and wild forms, differing in plant and fruit size and fruit composition, can be found. These differences may have resulted from the domestication of the cultivated form over a long period of time. We investigated whether wild and cultivated forms of myrtle differ in karyological features (i.e., number of somatic chromosomes and relative genome size). We sampled two wild forms and six cultivated types of myrtle. All the samples had the same chromosome number (2n = 2x = 22). The results were confirmed by 4',6-diamidino-2-phenylindole (DAPI) flow cytometry. Only negligible variation (similar to 3%) in relative fluorescence intensity was observed among the different myrtle accessions, with wild genotypes having the smallest values. We concluded that despite considerable morphological differentiation, cultivated and wild myrtle genotypes in Turkey have similar karyological features.Öğe Relationships among Crataegus accessions sampled from Hatay, Turkey, as assessed by fruit characteristics and RAPD(Springer, 2011) Serce, S.; Simsek, O.; Toplu, C.; Kamiloglu, O.; Caliskan, O.; Gunduz, K.; Ozgen, M.The genus Crataegus known as hawthorns, is the largest genus among the Maloideae, which comprises 265 species. Turkey is one of the genetic centers of Crataegus and there are more than 20 species found in Turkey. The fruits of Crataegus are used as food and have high flavonoid, vitamin C, glycoside, anthocyanidin, saponin, tannin, and antioxidant levels. In this study, we attempted to characterize 15 Crataegus accessions sampled from Hatay, located in Eastern Mediterranean region of Turkey. The accessions belonged to several species; C. aronia (L.) DC. var. aronia, C. aronia var. dentata Browicz, C. aronia var. minuta Browicz, C. monogyna Jacq. subsp. azarella (Griseb.) Franco, and C. orientalis Pall. ex M. Bieb. var. orientalis. Fruit characteristics of the accessions exhibited considerable variation. The multivariate, principle component and cluster analyses indicated that the accessions belonged to three groups: (1) C. aronia var. arona accessions; (2) C. aronia var. dentata accessions; and, (3) C. monogyna subsp. azarella and C. orientalis var. orientalis accessions. The principle component analysis results also revealed that the first three components explained 46, 21, and 14% of the variation, comprising a total of 81%. The fruit length and width, leaf area, and soluble solids contents were highly correlated characteristics for the first three components. The 19 RAPD primers generated a total of 107 bands, where 76 of these were polymorphic. The molecular data analyses by principle coordinate and clustering showed similar results to those of pomological characteristics. There were three groups, (1) C. aronia var. arona accessions; (2) C. aronia var. dentata accession; and, (3) C. monogyna subsp. azarella. C. orientalis var. orientalis accession grouped with C. aronia var. arona accessions. Therefore, it can be concluded that, overall, the diversity patterns of pomological and molecular data, generated by RAPD, for Crataegus are in good agreement and the accessions of C. aronia var. aronia, C. aronia var. minuta, C. monogyna subsp. azarella and C. orientalis var. orientalis accessions.Öğe Using General and Specific Combining Ability to Further Advance Strawberry (Fragaria sp.) Breeding(Int Soc Horticultural Science, 2014) Mathey, M. M.; Mookerjee, S.; Mahoney, L.; Finn, C. E.; Hancock, J. F.; Serce, S.; Davis, T.Strawberry is one of the five fruit crops included in the USDA-funded multi-institutional and trans-disciplinary project, RosBREED: Enabling Marker-Assisted Breeding in Rosaceae. A Crop Reference Set (CRS) was developed of 900 genotypes and seedlings from 40 crosses representing the breadth of relevant diversity and encompassing founders used in breeding the domesticated strawberry. Individual native species and cultivar genotypes were included along with 10 progeny from 36 of the crosses of genotypes representing eastern and western North American and European short day and remontant cultivars. This CRS has been phenotyped in five U.S. states. Over 14 fruit quality traits have been studied, as well as remontancy, truss size, peduncle length, crop estimate, plant architecture, and disease resistance. The phenotyping conducted in the first growing season showed considerable variability amongst the genotypes and the locations for all of the characteristics. General and specific combining ability variance components were determined from the populations in order to provide breeders with guidance on the most effective breeding strategies for incorporating the superior traits from this germplasm into their programs.
