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    Molecular detection of ‘Candidatus Phytoplasma mali’ associated with virescence in Narcissus tazetta in Turkey
    (Technology Society of Basic and Applied Sciences, 2023) Boztas, Kadir; Kocabag, Hamide Deniz; Derecik, Kayhan; Gazel, Mona; Sipahioglu, Hikmet Murat; Caglayan, Kadriye; Tulum, Isil
    In the winter 2019 virescence, flower sterility, and stunting symptoms were observed in daffodil plants in Mersin province of Turkey. To detect the presence of phytoplasmas ten plants showing symptoms and ten asymptomatic plants were assayed by PCR analysis. The nested-PCR analysis of extracted DNA with universal primer pairs amplifying 16S phytoplasma ribosomal gene were positive for 9 out of the 10 symptomatic daffodil samples. BLAST analysis of the 16S rDNA sequences, virtual restriction fragment length polymorphism (RFLP) and RFLP analyses confirmed the presence of ‘Candidatus Phytoplasma mali’ in Narcissus tazetta plants. The detected strains of ‘Ca. P. mali’ were sequenced and compared in multiple alignments using sequences in NCBI GenBank. The nucleotide identity among the 16S rDNA gene fragments ranged between 99.26 and 99.91% to ‘Ca. P. mali’, ribosomal subgroup 16SrX-A. Although phytoplasmas in this subgroup have been reported frequently from woody plants, this is the first report of a ‘Ca. P. mali’ strain infecting daffodil plants. © 2023, Technology Society of Basic and Applied Sciences. All rights reserved.
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    Molecular identification of phytoplasmas in ornamental pomegranates in Turkey
    (Technology Society of Basic and Applied Sciences, 2019) Çaglayan, Kadriye; Gazel, Mona; Acioglu, Mehtap; Kocabag, Hamide Deniz; Sipahioglu, Hikmet Murat
    Leaves, flowers and shoots of ornamental pomegranates showing yellowing and leaf deformation were collected from symptomatic and non-symptomatic plants between 2015 and 2017 from Hatay, Sanliurfa and Gebze provinces of Turkey. Among the 20 tested pomegranate samples, 10 were positive for phytoplasmas using 16R758f/16R1232r and U5/U3 primers amplifying 16S ribosomal RNA gene. The sequences of 8 strains shared the closest nucleotide identity (99%) with phytoplasmas in group 16SrIX and the remaining 2 strains had 98% identity with the ‘Candidatus Phytoplasma solani’ (subgroup 16SrXII-A). The present study represents the first evidence of phytoplasma association with yellowing symptoms in ornamental pomegranates in Turkey. © 2019, Technology Society of Basic and Applied Sciences. All rights reserved.