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Öğe Biofungicidal activities of plant essential oils against cucumber root and stem rot disease caused by fusarium oxysporum f. sp. radicis-cucumerinum(Edizioni ETS, 2017) Soylu, E.M.; Incekara, R.The aim of this study was to investigate effects of essential oils of origanum (Origanum onites L.), lavender (Lavandula stoechas L. subsp. stoechas), fennel (Foeniculum vulgare Mill.), laurel (Laurus nobilis L.) and myrtle (Myr-tus communis L.) against root and stem rot of cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) in vitro and in vivo. Inhibitory effects of contact and volatile phases of essential oils on mycelial growth and conidial germination were determined at various concentrations. Results clearly showed that those essential oils had a strong detrimental effect on mycelial growth and spore germination in a concentration-dependent manner. Essential oil of O. onites was strongly inhibitory to Forc as compared with essential oils of F. vulgare, L. stoechas, L. nobilis and M. communis. Volatile phases of essential oils were consistently found to be more effective on inhibition of mycelial growth and spore germination at lower concentrations in comparison to contact effect of the oils tested. Light and scanning electron microscopic (SEM) observations on inhibited fungal hyphae revealed considerable morphological degenerations on the fungal hyphae. Essential oil of O. onites, being the most efficient essential oil in vitro, was further evaluated under greenhouse conditions. The essential oil of O. onites displayed potent in vivo biofungicidal activity against the Forc on greenhouse-grown cucumber plants. Results of in vitro and in vivo studies demonstrated that the O. onites essential oils could be used as natural biofungicide to control root rot disease caused by Forc on cucumber. © 2017, Edizioni ETS. All rights reserved.Öğe Chemical composition and antibacterial activity of essential oils of bitter fennel (Foeniculum vulgare Mill. var. vulgare) and dill (Anethum graveolens L.) against the growth of food-borne and seed-borne pathogenic bacteria(Codon Publications, 2009) Soylu, Soner; Soylu, E.M.; Evrendilek, G.A.In the present study, the chemical composition and antibacterial activity of essential oils of bitter fennel [Foeniculum vulgare Mill. var. vulgare) and dill [Anethumgraveolens L.) were investigated against food-borne and seed- borne pathogenic bacteria. The chemical composition of bitter fennel and dill essential oils was analyzed by gas chromatography-mass spectroscopy. The major compounds found in the essential oils of bitter fennel and dill were estragole (37.6%) and limonene (33.1%), respectively. These essential oils have antibacterial activity against food-borne as well as seed-borne pathogenic bac-teria. Fennel essential oil had the highest antibacterial activity against the food-borne bacterial pathogen Staph- ylococus aureus, while dill essential oil had the highest antibacterial activ-ity against seed-borne pathogenic Cla- vibacter michiganensis subsp. michi- ganensis. Amongst all the bacterial species tested, the plant pathogenic agent Pseudomonas syringae pv. tomato had the greatest resistance to both essential oils. Considering the significant level of bacterial growth inhibition of seed-borne and food-borne pathogens, essential oils or their components could be promising seed disinfectants or food additives in appropriate products.Öğe Effect of pulsed electric fields on germination tube elongation and spore germination of Botrytis cinerea inoculated into sour cherry juice, apricot and peach nectars(Codon Publications, 2009) Akdemir Evrendilek, G.; Tok, F.M.; Soylu, E.M.; Soylu, SonerInactivation of Botrytis cinerea inoculated into sour cherry juice, apricot and peach nectars was determined by pulsed electric fields (PEF) based on the measurement of germination tube elongation, spore germination rate and light and scanning electron (SEM) mi-croscopic observations. With increasing electric field strength and processing time, germination tube elongation and spore germination were completely inhibited (P?0.05). Microscopic observations revealed considerable morphological alterations such as cytoplasmic coagulation, vacuolations, hyphal shriveling and protoplast leakage. PEF processing of juice/nectars was very effective to inactivate B. cinerea. This method could be used to prevent product loss in fruit juice/nectars due to B. cinerea contamination.Öğe Induction of disease resistance and antioxidant enzymes by acibenzolar-S-methyl against bacterial canker (Clavibacter michiganensis subsp. michiganensis) in tomato(2003) Soylu, E.M.; Soylu, Soner; Baysal, Ö.The plant defence activator acibenzolar-S-methyl (Bion) was assayed on tomato seedlings for its ability to induce multicomponent defence response against Clavibacter michiganensis subsp. michiganensis (Cmm). Pretreatment of plants with ASM reduced the severity of the disease as well as the growth of the bacteria in planta. In ASM-treated plants, reduction in disease severity (up to 75%) was correlated with the suppression of bacterial growth (up to 68.2%) during the time course of infection. In plants treated with ASM, activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione S-transferase (GST) were estimated as markers of resistance. The results indicated that ASM treatment led to enhanced activities of SOD and GST in tomato leaves. A slightly increase in SOD and GST activities was also found in Cmm-infected leaves. However, the increase in enzyme activities occurred much more rapidly and was more strongly enhanced in Cmm-infected leaves that were previously treated with ASM. The involvement of both enzymes in ASM mediated resistance in tomato is discussed.