Yazar "Türkez, Hasan" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ N/A ]
    Öğe
    Genotoxic effect of beryllium chloride and protective role of crataegus oxyacantha L. (Hawthorn) fruit extract in vitro
    (2012) Dirican, Ebubekir; Türkez, Hasan
    Beryllium (Be) is used in various industries (electronics, energy, automotive, and aerospace applications); however, there is very scarce data on its genotoxicity. On the other hand, Crataegus oxyacantha is a thorny tree that thrives in hedgerows and fields in the temperate regions of Europe and the British Isles. Besides, C. oxyacantha berry extract is known as one of the most powerful antioxidants. In this study, antigenotoxic effects of C. oxyacantha against Be-induced chromosomal aberrations (CA), sister chromatid exchanges (SCE) and micronucleus (MN) formation in human lymphocytes were investigated. All cultures were treated with 120 mg/L beryllium chloride (BeCl2) for 48 h. Three different concentrations (25, 50 and 100 mg/L) of C. oxyacantha fruit extract was added to the BeCl2-treated cultures. In BeCl 2 and C. oxyacantha combination, the frequency of abnormal cells and CA/cell significantly decreased compared to BeCl2 alone. Statistically significant reduction was also observed in SCE/cell and MN frequencies in all the treatments. These results illustrated anticlastogenic and antimutagenic effects of C. oxyacantha fruit extract against BeCl 2-induced genetic damage. On the other hand, BeCl2 + C. oxyacantha treatments induced significant reduction in the mitotic index, with regard to BeCl2 treatment alone. Based on the results of this study, it may be concluded that dose-controlled C. oxyacantha diet may play a protective role in the process of BeCl2 mutagenesis and/or carcinogenesis.
  • [ N/A ]
    Öğe
    Thymbra spicata l. modulates chromium (iii) chloride-induced genetic and oxidative damage in vitro
    (2012) Dirican, Ebubekir; Türkez, Hasan
    Chromium is a toxic heavy metal used in various industries and leads to environmental pollution due to improper handling. Cr exhibits oxidative stress mediated genotoxic damage although the mechanism of cellular damage caused by Cr has not been fully elucidated. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, was recently investigated popularly of biological roles; mainly antioxidant and antimicrobial activities. However, there is very scarce data on the cytogenetic effects of Thyme species. Therefore, in this study, we aimed to determine whether T. spicata extracts conferred a protection against Chromium (III) chloride (CrCl3)-induced genotoxic and oxidative damage in vitro. For this aim, we determined sister chromatid exchange (SCE) rates and main antioxidant enzyme activities including catalase (CAT) superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in CrCl 3 (25 ?M) and T. spicata extracts (TSE) (25, 50, 100 ?M) treated human whole blood cultures (n=3) for 72h. T. spicata extracts at tested concentrations did not exhibit any negative effects on above studied parameters in culture tubes. Besides, the results of the present study indicated that the increases of SCE frequencies and the decreases of antioxidant enzyme activities by CrCl3 were minimized by the application of the T. spicata extracts. Our results firstly suggest that T. spicata augments the antioxidants defense against CrCl3 induced toxicity. Based on the results of this study, it may be concluded that dose controlled T. spicata diet may play a protective role in the process of Cr mutagenesis and/or carcinogenesis.