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Öğe Alteration of nitric oxide production in rats exposed to a prolonged, extremely low-frequency magnetic field(Taylor & Francis Inc, 2007) Akdag, M. Zulkuf; Bilgin, M. Hakki; Dasdag, Suleyman; Tumer, CemilThe purpose of this stud), is to investigate the possible effect of an extremely low frequency magnetic field (ELF-MF) on nitric oxide (NO) level. In this study, 27 male Sprague-Dawley rats were used. The rats were divided into three groups: two experimental and one control (sham-exposed). The first and second experimental group (n = 10) were exposed to 100 mu T and 500 mu T ELF-MF during 10 months, 2 h a day, respectively, and the third (n = 7) group was treated like an experimental group except for ELF-MF exposure in methacrylate boxes. After ELF-MF and sham exposure, serum nitrite levels were measured by Griess reaction. A significant reduction was observed in nitrite levels among the first and second experimental groups of rats and sham-exposed rats after exposure for 10 months, 2 h a day, to ELF-MF of 100 and 500 LT (p < 0.01). These results suggest that prolonged ELF-MF exposure at intensities of exposure limits, determined by ICNIRP for public and occupational, may reduce NO production probably affected by NO generation pathways.Öğe Anti-proliferative Effects of Trigonella foeniculum and Eucalyptus camaldulensis on Human Melanoma Cells(Wiley, 2017) Duran, Nizami; Duran, Gulay Gulbol; Kaya, D. Alpaslan; Ay, Emrah; Tumer, Cemil; Demir, Enver Ahmet[Abstract Not Available]Öğe Anticonvulsive and behavior modulating effects of sophoretin and rutoside(Akademiai Kiado Zrt, 2019) Demir, Enver Ahmet; Ozturk, Atakan; Tutuk, Okan; Dogan, Hatice; Tumer, CemilIntroduction: Seizures are the hallmarks of most types of epilepsies. Behavioral and cognitive impairments coincide with interictal periods even though it is not clear whether these impairments spring out of the seizure itself or accompanying sociopsychological burden of the disease. Materials and methods: In this study, we investigated behavioral and cognitive consequences of a single GABA receptor-related seizure in mice, and examined the potential anticonvulsive and behavior-modulating properties of sophoretin (quercetin) and rutoside (rutin). Results: The study demonstrated that sophoretin and rutoside, common flavonoids of the human diet, delay the seizure onset and reduce the seizure stage. Moreover, they exerted an antidepressant-like effect, which was independent of the seizure. Neither treatments nor seizure altered recognition and spatial memory performances of the mice. Conclusions: Behavioral or cognitive disturbances that are evident in epileptic patients did not appear following a single seizure. In addition, we suggest that both sophoretin and rutoside successfully alleviate the seizure severity without interfering in the behavioral stability and cognitive performance. Hence, these flavonoids may be of use as adjuncts to the current treatment options.Öğe Antiepileptic effects of ghrelin on pentylenetetrazole-induced seizures in rats(Elsevier Science Inc, 2007) Obay, Basra Deniz; Tasdemir, Ezel; Tumer, Cemil; Bilgin, Hakki Murat; Sermet, AbdurrahmanIt is well known that neuropeptide Y (NPY) and gamma-aminobutyric acid (GABA) exert antiepileptic effects in animal models. It has recently been shown that ghrelin neurons increase the activities of GABA and NPY in the brain. Therefore it can be said that ghrelin is an antiepileptic agent. In this study we aimed to investigate the antiepileptic effect of ghrelin in an acute experimental epilepsy model in pentylenetetrazole (PTZ) injected rats. Adult male Wistar albino rats were divided into a control group and four experimental groups with seven rats in each group. In order to generate epileptic seizures, PTZ (50 mg/kg) was injected intraperitone ally. The experimental groups received intraperitoneal injections of ghrelin at doses of 20,40, 60 and 80 mu g/kg 30 min before PTZ injection. After PTZ injection, the latencies were separated into three components: first myoclonic jerk, generalized clonic seizures and tonic generalized extension. The injection of 50 mg/kg PTZ-induced epileptic seizures in the control group. The onset-times of the three characteristic behavioral changes were significantly delayed and the duration of tonic generalized extension was diminished by dose-dependent ghrelin administration. Our results demonstrated that ghrelin suppresses the onset time of PTZ-induced seizures. In the light of our current knowledge, it seems that ghrelin may be considered as an antiepileptic drug. (c) 2007 Elsevier Inc. All rights reserved.Öğe The association of endothelial nitric oxide synthase gene G894T polymorphism and serum nitric oxide concentration with microalbuminuria in patients with gestational diabetes(Dustri-Verlag Dr Karl Feistle, 2014) Atay, Ahmet Engin; Akbas, Halit; Tumer, Cemil; Sakar, Mehmet Nafi; Esen, Bennur; Incebiyik, Adnan; Simsek, SeldaAim: Gestational diabetes mellitus (GDM) is a glucose intolerant condition that affects 14% of all pregnancies. Diabetes mellitus (DM) occurs in 30 - 70% of patients with GDM after delivery. DM and GDM are associated with structural and functional deterioration of the renovascular system. Our aim is to investigate the association Glu-298Asp polymorphism of the endothelial nitric oxide synthase (eNOS) gene with serum nitric oxide levels and microalbuminuria in patients with GDM and healthy pregnancies. Material and methods: Serum nitric oxide (NO) levels, urinary excretion of albumin and Glu298Asp polymorphism of the eNOS gene were analyzed in 68 patients with GDM and 73 healthy controls. High performance liquid chromatography (HPLC-Griess) method was used to analyze serum NO levels. Microalbuminuria was evaluated by rate nephelometry method. The Glu298Asp polymorphism of the eNOS gene was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: Nitric oxide, glucose, creatinine, and microalbuminuria were significantly different between the patients and the control subjects (p = 0.001, p = 0.001, p = 0.002, and p = 0.005, respectively). There was a significant difference between groups in terms of the ratio of GG/GT+TT of eNOS gene Glu298Asp (p = 0.02). The patients with GT+TT genotype had significantly higher microalbuminuria levels and lower NO concentrations (22.16 vs. 9.51, p = 0.005, and 10.56 vs. 12.73, p = 0.021, respectively). The presence of T allele of eNOS gene is an independent predictor of microalbuminuria (OR: 2.346, 95% confidence interval: 1.247 - 5.238, p = 0.02) as well as serum glucose and NO concentration. Conclusion: The G894T polymorphism of eNOS gene and decreased NO concentration seem to be independent predictors of increased urinary excretion of albumin in patients with GDM. Determining the frequency of eNOS gene G894T polymorphism may help to identify pregnancies at increased risk of microalbuminuria.Öğe Association with Leptin Gene c.-2548 G>A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients(Humana Press Inc, 2013) Sahin, Deniz Say; Tumer, Cemil; Demir, Cemil; Celik, M. Murat; Celik, Mustafa; Ucar, Edip; Gunesacar, RamazanLeptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G > A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G > A polymorphism were detected using polymerase chain reaction-restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G > A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %) when compared with controls (14.6, 12.5, and 27.1 %; p = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels (p < 0.001) and body mass index (p < 0.0001). All these consequences showed that LEP -2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population.Öğe The Comparison of Visceral Adiposity Measures Between Type 2 Diabetics and Healthy Individuals(Wiley, 2018) Egeli, Duygu; Demir, Enver Ahmet; Dogan, Hatice; Tutuk, Okan; Acik, Murat; Tumer, Cemil[Abstract Not Available]Öğe Determining the Ideal Object Size in the Novel Object Recognition Test(Wiley, 2019) Kasirga, Busra; Demir, Enver Ahmet; Dogan, Hatice; Tutuk, Okan; Tumer, Cemil[Abstract Not Available]Öğe Dose dependent effects of ghrelin on pentylenetetrazole-induced oxidative stress in a rat seizure model(Elsevier Science Inc, 2008) Obay, Basra Deniz; Tasdemir, Ezel; Tumer, Cemil; Bilgin, Hakki Murat; Atmaca, MukadderIt has been suggested that free oxygen radicals play a role in the genesis of epilepsy and in post-seizure neuronal death. The aim of this study was to investigate the dose dependent effect of ghrelin on pentylenetetrazole (PTZ)-induced oxidative stress in a rat seizure model. For this purpose, the ghrelin groups were treated with intraperitoneal injections of ghrelin at doses of 20, 40, 60 and 80 [mu g/kg before the PTZ injection. Superoxide dismutase (SOD) and catalase (CAT) activities, and reduced glutathione (GSH) and thiobarbituric acid-reactive substance (TBARS) levels were measured in erythrocytes, liver and brain tissue. TBARS, the indicator of lipid peroxidation, was significantly increased in erythrocytes, liver and brain tissue, while antioxidant enzyme activities and glutathione levels were significantly decreased in PTZ injected rats. Ghrelin pretreatment prevented lipid peroxidation and the reduction in antioxidant enzyme activities and GSH levels against PTZ-induced oxidative stress in a dose dependent manner. The present data indicates that PTZ at a convulsive dose induces an oxidative stress response by depleting the antioxidant defense systems and increasing lipid peroxidation in the erythrocytes, liver and brain of rats. Ghrelin pretreatment diminished oxidative stress and prevented the decrease in antioxidant enzyme activities, and thus may reduce neuronal death in the brain during seizures. However, further studies are needed in order to confirm our hypothesis. (c) 2007 Elsevier Inc. All rights reserved.Öğe Effect of Alpha-LipoicAcid on TRPM7 Gene Expression in Experimental Varicocele Model(Wiley, 2018) Tutuk, Okan; Dogan, Hatice; Demir, Enver Ahmet; Tutuk, Sumeyye; Tumer, Cemil[Abstract Not Available]Öğe The Effect of Caffeic Acid Phenethyl Ester (CAPE) on TRPM 2, 8 Channels in Hepatic Ischemia-Reperfusion Model(Wiley-Blackwell, 2016) Dokuyucu, Recep; Dogan, Hatice; Tutuk, Okan; Sefil, Fatih; Gogebakan, Bulent; Gokce, Hasan; Tumer, Cemil[Abstract Not Available]Öğe The Effect of Chia (Salvia hispanica L.) Seed in an Experimental Alzheimer's Disease Model(Wiley, 2018) Bilgic, Yasemin; Demir, Enver Ahmet; Bilgic, Nilufer; Dogan, Hatice; Tutuk, Okan; Tumer, Cemil[Abstract Not Available]Öğe Effect of ghrelin on gastric myoelectric activity and gastric emptying in rats(Elsevier, 2008) Tumer, Cemil; Oflazoglu, Hueda Diken; Obay, Basra Deniz; Kelle, Mustafa; Tasdemir, EzelGhrelin is a recently discovered peptide in the endocrine cells of the stomach, which may stimulate gastric motility via the vagal nerve pathway. However, the mechanism of ghrelin-induced changes in gastrointestinal motility has not been clearly defined. The purpose of this study was to investigate the pharmacological effects of ghrelin on gastric myoelectrical activity and gastric emptying in rats, and to investigate whether cholinergic activity is involved in the effects of ghrelin. The study was performed on Sprague-Dawley rats implanted with serosal electrodes for electrogastrographic recording. Gastric slow waves were recorded from fasting rats at baseline and after injection of saline, ghrelin, atropine, or atropin+ghrelin. Gastric emptying of non-caloric liquid was measured by the spectrophotometric method in conscious rats. Intravenous administration of rat ghrelin (20 mu g/kg) increased not only dominant frequency, dominant power and regularity of the gastric slow wave but also the gastric emptying rate when compared with the control rats (P < 0.01, P < 0.05, P < 0.05, P < 0.001 respectively). These stimulatory actions of ghrelin on both gastric myoelectrical activity and gastric emptying were not fully eliminated by pretreatment with atropine sulphate. These results taken together suggest that ghrelin may play a physiological role in the enteric neurotransmission controlling gastric contractions in rats. (c) 2007 Elsevier B.V. All rights reserved.Öğe Effect of mesalamine on healing in experimental colon anastomosis: A randomised experimental study(2008) Aslan, Ahmet; Temiz, Muhyittin; Hakverdi, Sibel; Polat, Gurbuz; Tumer, Cemil; Temiz, Abdulkerim; Canbolant, ElifObjective: We aimed to investigate the effect of mesalamine on healing of experimental colon anastomosis model. Material/Methods: Forty adult male Wistar albino rats were performed segmentary colonic resection and end-to-end anastomosis. Animals were randomly divided into four groups: group I, anastomosis group, received no treatment (GI, n = 8); group II, anastomosis + oral mesalamine group (100 mg/kg/day); group III, anastomosis + rectal mesalamine (2 mL) group, (GIII, n = 8); group IV, anastomosis + oral mesalamine + rectal mesalamine (GIV, n = 8) group. A sham group (n = 8) was constituted and was performed laparotomy. Bursting pressure, hydroxyproline levels and histopathological characteristics of the anastomosis were analyzed. Results: Although it was not statistically significant, there was an increase in the burst pressure of the mesalamine group. When hydroxyproline measurements were compared there were statistically significant difference between the non-treated colon and all groups. There were significant differences between GI and GIII-GIV, GII and GIV. The differences between group I and II and group II and III were not statistically significant. When we compared the median amount of the histopathological changes, we found significant difference between the anastomosis and the mesalamine groups (P < 0.05). But when mesalamine groups were compared with each other we did not observe a significant difference. Conclusion: Mesalamine had positive effects which were not statistically significant on bursting pressure and statistically different significant effects on hydroxyproline (HP) levels based on the way of administration and statistically significant positive effects on histopathologic anastomotic healing in experimental anastomosis model. © 2007 Surgical Associates Ltd.Öğe Effect of nitric oxide on phagocytic activity of lipopolysaccharide-induced macrophages(Wiley, 2007) Tumer, Cemil; Bilgin, Hakki Murat; Obay, Basra Deniz; Diken, Huda; Atmaca, Mukadder; Kelle, MustafaAmong the antimicrobial mechanisms associated with macrophages, NO produced by iNOS plays a major role in intracellular killing, but the relationship between NO and phagocytic activity after injection of inflammatory agents into the peritoneal cavity is not clear. The aim of the present study was to investigate the effect of nitric oxide (NO) on macrophage function after treatment with intraperitoneal lipopolysaccharide (LPS) and the role of exogenous L-arginine administration in this event. Six experimental groups and one control group, each consisting of seven Wistar rats were used: Group I: Control; Group II: LPS; Group III: LPS + L-arginine; Group IV: LPS + L-arginine + Aminoguanidine; Group V: LPS + Aminoguanidine; Group VI: L-arginine; Group VII: Aminoguanidine. Macrophage phagocytic activity and total plasma nitrite levels were increased in the LPS group. In the LPS + L-arginine group, both the phagocytic activity and total plasma nitrite levels showed large increases. Administration of aminoguanidine (AG), a specific iNOS inhibitor, abolished macrophage phagocytic activity and total plasma nitrite levels in the LPS and LPS + L-arginine groups. As a result, we showed that NO produced by macrophages has a role not only in intracellular killing, but also in phagocytic activity. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.Öğe Effect of thymoquinone on transient receptor potential melastatin (TRPM) channels in rats with liver ischemia reperfusion model in rats(Mashhad Univ Med Sciences, 2024) Caglar, Kerem; Dokuyucu, Recep; Agturk, Gokhan; Tumer, Cemil; Tutuk, Okan; Gocmen, Hatice Dogan; Gokce, HasanObjective(s): We aimed to investigate the levels of transient receptor potential melastatin (TRPM) gene expression, and the antioxidant and histopathologic effect of thymoquinone (Tmq) in the hepatic I/R rat model. Materials and Methods: Fifty Wistar rats were divided into 5 groups. Group 1: Control; Group 2: Sham; Group 3: Hepatic I/R (45 min/45 min); Group 4: Tmq (50 mg/kg); Group 5: Tmq+I/R (ten days before I/R at the dose of 50 mg/kg of Tmq). The hepatic I/R (45min/45min) model was performed at the portal vein and the hepatic artery with atraumatic vascular clamp in the ischemia groups. The liver tissues and blood samples that were taken at the end of the study were evaluated for histopathologic and biochemical analysis. Besides TRPM gene expression levels were determined in liver tissues. It was seen that cellular swelling, congestion, PNL, and apoptosis parameters statistically decreased in Tmq and Tmq+I/R groups in comparison with the I/R group in histopathological evaluation. Results: It was observed that biochemical parameters, AST, ALT, GGT, LDH, creatinine, and urea levels significantly increased in the I/R group as compared with, sham, Tmq, and Tmq+I/R groups. It was found that TRPM2,6,7,8 gene expression decreased significantly in Tmq+I/R groups as compared to the I/R group. Conclusion: We showed that thymoquinone can inhibit the entry of Ca+2 into the cell by decreasing TRPM2,6,7,8 gene expression. Based on our findings, we think that Tmq application in the treatment of liver diseases due to I/R damage may be important in terms of both ischemia and apoptosis and can also be used in the treatment of liver-related diseases.Öğe The Effect of Vitamin D in an Experimental Varicocele Model(Wiley, 2018) Agturk, Gokhan; Demir, Enver Ahmet; Tutuk, Okan; Dogan, Hatice; Ozgur, Tumay; Tumer, Cemil[Abstract Not Available]Öğe Effect of vitamin D in experimental varicocele model in rats(Cukurova Univ, Fac Medicine, 2024) Agturk, Gokhan; Tumer, Cemil; Tutuk, Okan; Gocmen, Hatice Dogan; Ozgur, Tumay; Ozcan, Oguzhan; Duran, Gulay GulbolPurpose: Varicocele is a pathological condition that affects approximately 20% of adult men, causing infertility and sperm deterioration. The aim of our study was to investigate the efficacy of vitamin D (Vit. D) in the pathophysiology of varicocele. We performed biochemical, gene expression analyses and histopathological to evaluate the efficacy of vitamin D in the experimental varicocele model. Materials and Methods: In the study, 30 adult male Wistar Albino rats were used. The rats were divided into 3 groups equally as control group, experimental group (varicocele), treatment group (varicocele + D vit.). The treatment group received 500 IU/kg D vit. intramuscularly. Results: Histopathological, TRPM2-8 gene expression and biochemical analyses were performed on testicular and blood samples collected at the end of the experiment. The experimental group showed a deterioration in tubular structure, a decrease in total antioxidant levels and an increase in total oxidant levels. The treatment group, on the other hand, showed an increase in TAS, a decrease in TOS and a beneficial improvement in tubular structure disorders. Analysis of gene expression levels showed that TRPM2-8 expression levels were significantly increased in the varicocele group and decreased in the treatment group. Conclusion: In the varicocele model, the use of vitamin D had a significant effect on TRPM2-8 gene level, pathological seminiferous tubules and biochemical values. Further studies are needed to determine the clinical application of vitamin D in varicocele disease.Öğe The Effects of Erdosteine and Vitamin D on Liver Damage After Renal Ischemia Reperfusion(Wiley, 2017) Demir, Enver Ahmet; Dogan, Hatice; Tutuk, Okan; Ozcan, Oguzhan; Ozgur, Tumay; Tumer, Cemil[Abstract Not Available]Öğe The Effects of Erdosteine and Vitamin D on TRPM 2 Ion Channel in Renal Ischemia Reperfusion Damage Model in Rats(Wiley, 2018) Dogan, Hatice; Tutuk, Okan; Demir, Enver Ahmet; Tumer, Cemil[Abstract Not Available]
