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    Detection of fig mosaic virus in viruliferous eriophyid mite Aceria ficus
    (2012) Caglayan, K.; Elci, E.; Ulubas Serce, C.; Kaya, K.; Gazel, M.; Medina, V.
    Fig leaves showing typical fig mosaic symptoms on cv. Bursa siyahi{dotless} (donor plant) were cut under a stereo microscope into small pieces each hosting 10 putatively viruliferous eriophyid mites (Aceria ficus Cotte) and placed directly on the top leaves of healthy Cucumis sativus, Chenopodium quinoa. C. amaranticolor, Nicotiana occidentalis, Catharanthus roseus, Fraxinus excelsior plants, and fig seedlings. Donor and test plants were analyzed by electron microscopy, RT-PCR and sequencing, whereas the mites (ErMs) underwent molecular assays using Fig mosaic virus (FMV)-specific primers. Mite-infested leaves of fig seedlings and C. roseus showed small yellowish spots after 10 days and 6 weeks, respectively, whereas no symptoms were observed in other test or control plants for three months. Electron microscopy observations showed the occurrence of double membrane bodies (DMBs) in the palisade cells of donor and mite-inoculated fig plants, but not in C. roseus. However, 302 bp RT-PCR products specific to FMV were amplified from donor and inoculated figs, C. roseus and ErMs. Nucleotide identity with the sequence of the FMV isolate in GenBank (accession No. AM941711.6) was 87%, 89% and 87% for donor plant (JQ708183), inoculated fig seedlings (JQ708184) and C. roseus (JQ408437, JQ408438), respectively. The sequences obtained from ErMs (JQ408432, JQ408436) showed 87% and 88% nucleotide identity with the reference FMV isolate, respectively. When dsRNA extracts were analyzed to confirm virus presence in inoculated periwinkles, a complex dsRNA profile was obtained, suggestive of infection by a multipartite virus or by multiple viruses. Sequence from RT-PCR amplicons of dsRNA (JX040436) showed 88% identity with those the reference FMV isolate (AM941716.1) and the donor plant (JQ708183). According to these results, Madagascar periwinkle (C. roseus) can be retained as a new experimental host for FMV and A. ficus appears to be able to transmit FMV from fig to periwinkle plants.
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    Öğe
    Identification and Genomic Variability of Prune dwarf virus Variants Infecting Stone Fruit Trees in Turkey
    (Wiley, 2009) Ulubas Serce, C.; Ertunc, F.; Ozturk, A.
    Prune dwarf virus (PDV), a pathogen of stone fruit trees worldwide, shows a great variability in its biological, serological and molecular properties. The coat protein sequences of ten PDV variants from different stone fruit tree species in Turkey were determined. The sequence for each variant was 657 nucleotides in length. The phylogenetic analyses of the sequences of the Turkish variants and of additional sequences of other PDV variants from international databases indicate the existence of four groups of PDV variants: one contains cherry variants that differs in group specific conserved amino acids; a second contains one apricot and cherry variants, most members of the group being Turkish variants; a third contains mixed variants from cherry, peach, plum trees and an almond tree; and a fourth contains only variants from almond trees. Based on their hosts, we propose the nomenclature of these groups as cherry I, cherry II, mixed and almond groups, respectively.
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    Incidence and genetic diversity of peach latent mosaic viroid isolates in Turkey
    (2008) Gazel, M.; Ulubas Serce, C.; Caglayan, K.; Luigi, M.; Faggioli, F.
    In 2004 and 2005, 51 peach and 5 nectarine trees from different peach growing areas of the East Mediterranean Region of Turkey were analyzed by RT-PCR for the presence of Peach latent mosaic viroid (PLMVd). Most of the samples were from germplasm collections and did not show specific symptoms. PLMVd was detected in three nectarine and eight peach trees. The nucleotide sequence of the eleven PLMVd isolates was determined. Like previously studied PLMVd isolates, Turkish isolates varied in length from 336 to 340 nucleotides. Multiple alignments and phylogenic analyses showed that 31 clones of 11 isolates clustered in PLMVd Group III. Three clones of isolate 99 showed the greatest variability among the isolates characterized in this work and 12 previously characterized isolates. mFold analysis of secondary structures of the three clones of isolate 99 showed that most of the variations are located in a stem involved in the pseudoknot structure, in loop 10 and in the hammerhead arm region. PLMVd isolate 99 showed a high identity with isolate 162.4 from peach cv. Early May Crest.