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    Fatty acid, tocopherol, mineral composition, total phenolic, flavonoid, thymoquinone content, and antioxidant activity of Nigella Sativa L.
    (University of Ankara, 2014) Uras, S. Selma; Silahtaro?lu, Sibel; Ilçim, Ahmet; Kökdil, Gamze
    The fatty acid and tocopherol composition of fixed oil from seeds of Nigella sativa L. has been investigated by GC-MS and HPLC, respectively. Linoleic and oleic acids were the main fatty acids. The major tocopherols were ?- and ?-tocopherols. The total phenolic and flavonoid content and primary antioxidant activity of aqueous methanolic extracts of different parts of N.sativa were measured. Total phenolic content was assessed by Folin-Ciocalteu method and the content of flavonoids was measured spectrophotometrically by using the AlCl3 assay. Primary antioxidant activity in terms of free radical scavenging activity of the extracts was evaluated by DPPH assay. The present study showed that the seed extract contained significantly higher amount of total phenolic compounds than aerial part extract. The total flavonoid content ranged between 106.10±0.10 and 118.06±0.02 mg rutin equivalent per g extracts. The thymoquinone content in seeds were quantified by RP-HPLC analysis and the concentration ofthymoquinone was 268.3 ?g/mL. In DPPH radical scavenging assay, inhibition % of the extracts was found to be 95.09-90.71. The seeds also were analyzed by ICP-MS to determine mineral content. P, K, Ca and Mg were predominant elements among seventeen minerals in the seeds.