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Öğe Diz osteoartritli hastalarda eklem sıvısında nitrik oksit değerleri ve süperoksit dismutaz enzim aktiviteleri(2007) Kalacı, Aydıner; Uz, Efkan; Aslan, Bahadır; Söğüt, Sadık; Özkan, Cenk; Yanat, Ahmet NedimAmaç: Diz osteoartritli (OA) hastaların eklem sıvısında nitrik oksit (NO) düzeyi ve süperoksit dismutaz (SOD) enzim aktivitesi araştırıldı. Hastalar ve yöntemler: Amerikan Romatoloji Birliği OA ölçütlerine uyan 36 hasta (31 kadın, 5 erkek; ort. yaş 63; dağılım 48-88) çalışmaya alındı. Hastaların diz grafileri Kellgren-Lawrence radyografik sınıflamasına göre derecelendirildi. Sinovyal sıvı örnekleri, OA nedeniyle diz protezi veya hiyaluronik asit enjeksiyonu yapılan hastalardan girişimden önce iğne aspirasyonuyla toplandı, daha sonra Eppendorf tüplerine aktarılarak -80 °C’de saklandı. Sinovyal sıvıdaki nitrit ve nitrat değerleri Griess reaksiyonuna dayalı spektrofotometrik yöntemle belirlendi. Toplam SOD aktivitesi de spektrofotometrik yöntemle ölçüldü. Sonuçlar, diz eklemleri klinik ve radyografik olarak normal bulunan, ancak nedeni bilinmeyen diz ağrısından dolayı artroskopi uygulanan 10 hastayla (6 kadın, 4 erkek; ort. yaş 49; dağılım 26-70) karşılaştırıldı. Bulgular: Osteoartritli hasta grubunda NO düzeyi kontrol grubuna göre anlamlı derecede yüksek, SOD aktivitesi düşük bulundu (p<0.001). Kellgren-Lawrence radyografik derecelendirme grupları arasında NO ve SOD değerleri açısından anlamlı fark yoktu (p>0.05). Sonuç: Bulgularımız NO’nun kıkırdak yıkım mediyatörü, SOD’nin ise antioksidan mediyatör olduğunu düşündürmektedir. Bu değerlerin klinik önemini aydınlatmak için daha kapsamlı çalışmalara ihtiyaç vardır.Öğe Effects of hyaluronan on nitric oxide levels and superoxide dismutase activities in synovial fluid in knee osteoarthritis(Springer London Ltd, 2007) Kalacı, Aydıner; Yilmaz, H. Ramazan; Aslan, Bahadir; Soeguet, Sadik; Yanat, Ahmet Nedim; Uz, EfkanThe aim of the present study was to evaluate the effects of hyaluronan (HA) on nitric oxide (NO) levels and superoxide dismutase (SOD) enzyme activities in synovial fluid (SF) in the treatment of patients with knee osteoarthritis (OA). SF samples were aspirated from OA patients before the commencement of the treatment (n=23) and 6 weeks after they were treated with HA products. NO levels and SOD activities were compared between the pre- and post-treatment of OA patients and of the control group (n=10). SF NO levels were significantly higher in patients with OA before the commencement of the treatment compared with the post-treatment (p < 0.001) and the control groups. The SF SOD activity of patients before the commencement of the treatment was lower than the values in the controls and post-treatment (p < 0.001). There is no significant correlation between SF NO and SOD levels and the radiographic changes of the OA knee according to Kellgren-Lawrence grading (p > 0.05). Also, the Western Ontario and McMaster Universities Osteoarthritis index (WOMAC) pain scores and physical function scores were gradually improved. These findings made us think that SF NO was a potent mediator in cartilage damage in OA, whereas SOD was an antioxidant mediator in the same process. Exogenous HA injections might reduce the NO levels and increase SOD activities in synovial fluid. These effects also do not seem to be dependent on the radiographic grading of the OA knee. More comprehensive studies are needed to clarify a possible clinical significance of this topic, and we suggest that this is an important area for further research into new treatment options.Öğe Nitric oxide levels and superoxide dismutase enzyme activity in synovial fluid of patients with knee osteoarthritis(Turkish Joint Diseases Foundation, 2007) Kalacı, Aydıner; Uz, Efkan; Aslan, Bahadir; Soeguet, Sadik; Oezkan, Cenk; Yanat, Ahmet NedimObjectives: We investigated nitric oxide (NO) levels and superoxide dismutase (SOD) enzyme activity in synovial fluid of patients with primary knee osteoarthritis (OA). Patients and methods: The study included 36 patients (31 females, 5 males; mean age 63 years; range 48 to 88 years) with knee OA according to the diagnosis criteria of American College of Rheumatology. Radiographic severity of OA was assessed according to the Kellgren-Lawrence grading system. Synovial fluid samples were taken by needle aspiration before knee arthroplasty or hyaluronic acid injection and stored at -80 degrees C in Eppendorf tubes. Nitrite and nitrate levels were determined by the spectrophotometric method based on the Griess reaction. Total SOD activity was determined by the spectrophotometric method. The results were compared with those of 10 controls (6 females, 4 males; mean age 49 years; range 26 to 70 years) who clinically and radiographically had normal knees, but underwent arthroscopic examination for knee pain of unknown origin. Results: Compared to controls, NO levels were significantly higher and SOD activity was significantly lower in patients with OA (p < 0.001). No significant differences were found between radiographically graded groups with regard to NO level and SOD activity (p > 0.05). Conclusion: Our data suggest that NO acts as a potent mediator of cartilage damage and SOD as an antioxidant mediator in OA. Further studies are needed to clarify the clinical significance of these parameters.Öğe The protective effect of N-acetylcysteine against cyclosporine A-induced hepatotoxicity in rats(John Wiley & Sons Ltd, 2008) Kaya, Hasan; Koc, Ahmet; Sogut, Sadik; Duru, Mehmet; Yilmaz, H. Ramazan; Uz, Efkan; Durgut, RamazanThe immunosuppressive agent cyclosporine A (CsA) has been reported to exert measurable hepatotoxic effects. One of the causes leading to hepatotoxicity is thought to be reactive oxygen radical formation. The aim of this study was to investigate the effects of N-acetylcysteine (NAC) treatment on CsA-induced hepatic damage by both analysing superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aspartate aminotransferase (AST) and alanine transaminase (ALT) activities with malondialdehyde (MDA) and nitric oxide (NO) levels, and using an histological approach. CsA administration produced a decrease in hepatic SOD activity, and co-administration of NAC with CsA resulted in an increase in SOD activity. MDA and NO levels increased in the CsA group and NAC treatment prevented those increases. A significant elevation in serum AST and ALT activities was observed in the CsA group, and when NAC and CsA were co-administered, the activities of AST and ALT were close to the control levels. CsA treatment caused evident morphological alterations. Control rats showed no abnormality in the cytoarchitecture of the hepatic parenchyma. The co-administration of NAC with CsA showed no signs of alteration and the morphological pattern was almost similar to the control group. In conclusion, CsA induced liver injury and NAC treatment prevented the toxic side effects induced by CsA administration through the antioxidant and radical scavenging effects of NAC. Copyright (C) 2007 John Wiley & Sons, Ltd.
