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    Identification of Zygotic and Nucellar Individuals Produced from Several Citrus Crosses Using SSRs Markers
    (Univ Agr Sci & Veterinary Med Cluj-Napoca, 2013) Yildiz, Ercan; Kaplankiran, Mustafa; Demirkeser, Turan Hakan; Uzun, Aydin; Toplu, Celil
    Turkey is an important citrus-producing country. However, new cultivars are needed to sustain citrus production and ensure its competitiveness against other crops. There are currently several citrus breeding programs that aim to help overcome the lack of local commercial varieties and to contribute CO Turkey's competitive capacity in the citrus market. In this study, we report the utilization of molecular markers in one such breeding program. Simple sequence repeat (SSR) markers were employed to eliminate nucellar individuals from a hybrid population produced by crossing. The crosses included 'Fremont' and 'Robinson' mandarins as the female parents and 'Midknight Valencia', 'Rhode Red Valencia', and 'Valencia Late' oranges and 'Rio Red' grapefruit cultivars as the male parents. Seedlings with the same banding patterns as the female parent were identified as nucellar seedlings by 11 SSR primers. Primers AG 14 and TAA03 were found to be more effective at identifying zygotic individuals than other primers. 'Fremont' and 'Robinson' mandarins produced 36.91% and 31.09% of nucellar seedlings, respectively. As a pollen parent, 'Rio Red' grapefruit had a higher ratio of zygotic seedlings compared to 'Midknight Valencia', and can be recommended in breeding programs. Comparative analysis of different citrus fruits in the breeding programs allowed us to design an efficient hybridization scheme for this study.
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    Molecular characterization and genetic diversity analysis of mandarin genotypes by SSR and SRAP markers
    (Wfl Publ, 2013) Kacar, Yildiz; Uzun, Aydin; Polat, Ilknur; Yesiloglu, Turgut; Yilmaz, Bilge; Gulsen, Osman; Tuzcu, Onder
    The aim of the present study was to characterize the mandarin germplasm conserved at the Tuzcu Citrus Variety Collection at Cukurova University in Turkey using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) based molecular approaches. Genetic characterization of 65 mandarins was performed using 14 SSR markers and 21 SRAP markers. We originally tested 26 SSR markers, and 14 of these markers were selected due to their high polymorphism information content in the molecular assays. Thirty-eight alleles were detected at 14 loci. The number of alleles per SSR locus ranged from 2 to 4, with a mean of 2.7 alleles. The most informative loci were CAGG 9, CAT 01 and TAA 52 (4 alleles/primer). From the SRAP analysis, a total of 187 alleles were generated, and the polymorphism rate was 77%. The number of alleles detected by a single primer set ranged from 4 to 15, with an average of 8.90. The UPGMA dendrogram, defined by SSR and SRAP markers, revealed the genetic relatedness of the mandarin genotypes. These findings can be used to guide future breeding studies and germplasm management of these mandarin genotypes.