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    Antioxidant response to drought in red and white clover
    (Springer Heidelberg, 2012) Vaseva, Irina; Akiscan, Yasar; Simova-Stoilova, Lyudmila; Kostadinova, Anelia; Nenkova, Rosa; Anders, Iwona; Feller, Urs
    Antioxidant response to drought in red (Trifolium pratense L., cv. Start) and white clover (Trifolium repens L, cv. Haifa and cv. Debut) grown as soil cultures was evaluated in water-deprived and recovered plants. Drought provoked oxidative stress in leaves confirmed by the considerable changes in electrolyte leakage, malondialdehyde, hydrogen peroxides and proline contents. Immunoblot of Delta-1-pyrroline-5-carboxylate synthetase (P5CS), which catalyzes the first two steps in proline biosynthesis, revealed strong induction of the enzyme in red clover plants submitted to drought. Water-deprived white clover plants exhibited distinct P5CS profiles. This was related to different drought tolerance of the studied T. repens cultivars. Isoenzyme analyses of superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) demonstrated certain differences in antioxidant defence among the tested varieties. It was confirmed that MnSOD (in both T. repens and T pratense) and FeSOD (in T. repens) isoforms were the most affected by drought. The red clover cultivar Start exhibited the lowest FeSOD and POX activities which could contribute to its poor performance under water deprivation.
  • Küçük Resim
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    Drought stress tolerance of red and white clover-comparative analysis of some chaperonins and dehydrins
    (Elsevier, 2011) Vaseva, Irina; Akiscan, Yasar; Demirevska, Klimentina; Anders, Iwona; Feller, Urs
    The ecophysiological function linked to the contribution of the legume to the nitrogen supply of the sward, as well as its high content in pastures, make red (Trifolim protense) and white clover (Tnfolium repens) forage plants of economic importance in temperate regions. The study aimed to characterize the expression profiles of some important stress tolerance related proteins in red clover (cv. Start), and two white clover cultivars - cv. Haifa (medium-leafed) and cv. Debut (small-leafed). An attempt has been made to explain the differences in red and white clover potential to sustain unfavorable environmental conditions regarding the obtained results from protein and gene profiling. Rubisco binding protein (RBP), low molecular weight heat shock proteins (HSP), calpains (ClpA and ClpP) and dehydrins (DHN) responded significantly to drought. White clover cv. Debut developed the lowest leaf water deficit (WD) compared to the one detected in red clover and the medium leafed white clover cultivar Haifa. Immunoblot and RT-PCR showed that the small leafed white clover cv. Debut copes with drought stress most efficiently. Detailed gene expression analysis revealed that Y2SK type dehydrins were subjected to alternative splicing under drought and the transcripts were strongly induced in the more drought tolerant small-leafed white clover cultivar Debut. A homolog sequence of Y2K dehydrin in T. pro tense (GenBank ID: JF748409) and T. repens (GenBank ID: JF748410) have been amplified and an increased transcipt levels of JF748409 were detected in T. pratense drought stressed leaves. The expression pattern of SK2 transcripts showed that this dehydrin type has been down-regulated in both white clover cultivars under water deprivation. (C) 2011 Elsevier B.V. All rights reserved.