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Öğe Expression patterns and distribution of aquaporin water channels in cervix as a possible mechanism for cervical patency in bitches affected by pyometra(Elsevier Science Inc, 2024) Yazlik, Murat Onur; Ozkan, Hueseyin; Vural, Sevil Atalay; Kaya, Ufuk; Ozoner, Ozgur; Mutluer, Ipek; Altinbas, Yunus FurkanPyometra is a life-threatening disease, the severity of which depends on cervical patency status. This study investigated cervical inflammation status as well as the expression patterns and localization of aquaporin (AQP1, AQP2, AQP3, AQP5, and AQP9), and hormone receptors in cervical tissue that influences canine pyometra. Of the 36 animals enrolled in the study, 24 were diagnosed with pyometra and separated into two groups: open cervix pyometra and close cervix pyometra, while 12 healthy animals presented for elective ovariohysterectomies were allocated into the control group. Surgical treatment was performed for treatment of pyometra. After each operation, cervix samples were collected and analyzed for AQP and hormone receptor expression patterns determined by qPCR and protein expression by means of immunohistochemistry. Blood samples were also collected to determine serum progesterone concentrations. AQP9 expression was downregulated approximately 3-fold while and PGR expression was downregulated more than 2 fold in both pyometra groups compared to the control group. AQP3 and AQP5 gene expression levels were upregulated more than 3 fold in the open-cervix pyometra group than the closed-cervix pyometra group (P < 0.05). This is the first study to describe the expression patterns and immunolocalization of AQPs in canine cervical tissue based on pyometra patency status and to report AQP3 and AQP5 expression in cervical tissue linked to cervical patency.Öğe Expression patterns of inflammatory and oxidative stress-related genes in the uterine and ovarian tissues of dogs diagnosed with pyometra based on cervical patency status(Elsevier Science Inc, 2024) Ozkan, Huseyin; Yazlik, Murat Onur; Keceli, Hasan Huseyin; Vural, Sevil Atalay; Mustak, Inci Basak; Kaya, Ufuk; Altinbas, Yunus FurkanThis study aimed to investigate the expression patterns of genes associated with inflammation and oxidative stress in ovarian and uterine tissues of dogs with pyometra, categorized by cervical status (open cervix or closed cervix), which influences disease severity. The control group comprised healthy animals undergoing elective ovariohysterectomy. Tissue inflammatory gene expression and Malondialdehyde (MDA) levels were determined while microbial and histopathological examinations were conducted, along with immunohistochemical evaluations. In the closed-cervix group, uterine TNF and IL6 were upregulated approximately 10- fold while IL10 was upregulated nearly 5-fold. TNF expression differed remarkably between the pyometra groups. In the closed-cervix group, PTGS2 and HMOX1 were upregulated approximately 5-fold whereas NFE2L2 expression was downregulated. The closed-cervix group also had the highest uterine MDA levels. Regarding ovarian tissue, MDA levels were higher in the closed-cervix group than in the open-cervix group while IL10 expression was lower in the closed-cervix group than the open-cervix group. In the closed-cervix group, NFE2L2 was downregulated whereas HMOX1 was upregulated. Uterine TNF levels were positively correlated with IL6, , IL10, , PTGS2, , and HMOX1, , but negatively correlated with NFE2L2. . IL6 was positively correlated with IL10, , PTGS2, , and HMOX1. . NFE2L2 was negatively correlated with IL6 and HMOX1. . IL10 was positively correlated with PTGS2 and HMOX1. . MDA was positively correlated with TNF, , IL6, , IL10, , PTGS2, , NFE2L2, , and HMOX1. . TNF levels were positively correlated with ovarian PTGS2, , and with IL6 and NFE2L2. . MDA was positively correlated with PTGS2 and HMOX1. . MDA could be an important biomarker for understanding the severity of pyometra. Moreover, TNF expression and its relationships with various studied parameters such as IL10 may contribute to treatment and prognostic biomarker studies in closed-cervix pyometra pathology.Öğe Oropharyngeal tumors between 1977 to 2005 at dog and cats(Ankara Univ Press, 2007) Vural, Sevil Atalay; Keles, Hikmet; Oezsoy, Sule Yurdaguel; Oezkul, Ibrahim AyhanThe aim of the study was to evaluate dog (80 cases) and cat's (13 cases) oropharyngeal biopsy/operation materials, which were sent as tumor suspicion from Ankara University Veterinary Faculty Clinics and Private Clinics to Pathology Department between the years of 1977 to 2005. These tumors were frequently seen in dogs between 6-10 years, at the Terrier, Mongrel, Boxer, Shepherds breeds (Kangal and German), while cats were between 1-5 years, at the Tekir, Mongrel and Siamese breeds. Most of the dog tumors were diagnosed as epulides and papillomas (26.3%) and mostly affected regions were the gingiva (21.3%), submandibular area (17.5%) and lips (15%). Cat tumors were diagnosed as epulides (23.1%), plasmacytomas (15.4%) and other tumors (61.5%) and they were frequently located at-the submandibular area (38.5%), gingiva (30.8%) and palate (23.1%).Öğe Pleural mesothelioma in a nine-month-old dog(Veterinary Ireland, 2007) Vural, Sevil Atalay; Ozyildiz, Zafer; Ozsoy, Sule YurdagulThis paper reports on an unusual case of pleural epitheloid mesothelioma in a nine-month-old male, mixed breed dog. The dog was presented in-extremis and, on post mortem examination, multiple, exophytic, frequently pedunculated, yellowish-red, soft to firm masses ranging from 3mm to 6cm in diameter were diffusely distributed over, and attached to, the pericardial and parietal pleural surfaces. Microscopically, these masses consisted of round to partially polygonal-shaped, anaplastic cells with minimal cytoplasm and hyperchromatic nuclei covering papillomatous projections or as part of more densely cellular masses. A supporting fibrovascular stroma and mitotic figures were also evident. Constituent tumour cells were labeled positively with antibodies against both vimentin and cytokeratin. In contrast, the same cells exhibited equivocal labeling with an antibody directed against calretinin antigen and did not label with antibodies against carcinoembryonic antigen (CEA) and milk fat globule-related antigen (MFGRA). Such tumours are rare in dogs, particularly in such a young animal.Öğe The presence of virulence factor genes downregulates uterine AQP3 and alters glutathione peroxidase activity and uterine histopathology in canine pyometra(Wiley, 2024) Yazlik, Murat Onur; Mustak, Inci Basak; Ozkan, Hueseyin; Vural, Sevil Atalay; Kaya, Ufuk; Ozoner, Ozgur; Mutluer, IpekPresent study was designed to evaluate the role of virulence factor genes (papG, cnf1 and hylA) in the pathogenesis of canine pyometra. Antimicrobial susceptibility test and detection of virulence genes were performed Escherichia coli (E. coli) detected in uterine swab samples. Animals were divided into two groups based on the presence (VF+, n:14) or absence (VF-, n:7) of the virulence factor genes papG, cnf1 and hylA. Blood and tissue glutathione peroxidase activity, uterine histopathologic analysis and AQP3, ESR1, PGR, OXTR gene expressions were determined in both groups. Statistical analyses were performed using Stata version 15.1. All E. coli isolates were susceptible to amikacin, whereas resistant to ampicillin, amoxicillin/clavulanic acid and lincomycin. None of the isolates were susceptible to cefotaxime. E. coli isolates had at least one virulence gene. The most prevalent gene was fimH (100%), followed by fyuA (95.8%), usp (83.3%), sfa (75%), cnf1 and hlyA (70.8%) genes. Blood GPx activity was greater in VF+ animals. On the other hand, uterine tissue GPx activity was lower in VF+ group compared to the control group. Expression levels of AQP3 were upregulated more than fivefold in VF-dogs compared to the control group. In addition, AQP3 expression levels were found approximately threefold higher in VF (-) than VF (+) group (p < .05). Varying degree of inflammation noted for all animals with pyometra, but the presence of bacteria noted only in VF+ animals. In conclusion, the presence of virulence factor genes does not play a role in the histopathological degree of inflammation, the presence of bacteria was found to vary. Serum GPx activity increased in VF+ animals. While the hormone receptor expressions were similar, AQP expression was upregulated in the absence of virulence factor genes.
