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    Agrobacterium-mediated transformation of 'Kirkagac 637' a recalcitrant melon (Cucumis melo L.) cultivar with ZYMV coat protein encoding gene
    (Int Soc Horticultural Science-Ishs, 2004) Yalcin-Mendi, NY; Ipek, M; Serbest-Kobaner, S; Curuk, S; Kacar, YA; Cetiner, S; Gaba, V
    A gene encoding for zucchini yellow mosaic virus (ZYMV) coat protein was transformed into 'Kirkagac 637', an important Turkish melon cultivar using Agrobacterium tumefaciens, and putatively resistant transgenic fines were obtained. Proximal cotyledonary explants were incubated with Agrobacterium carrying the binary plasmid PGA643 including the nptii and zymv coat protein genes under the control of the CaMV 35S promoter. ELISA tests and PCR analyses were performed to determine which plants were transformants. Transformation efficiency was 1,6 %. Four transformants were self pollinated to obtain R-1 generation. The plants from the R-2 generation were tested by PCR to demonstrate the integration of the zymv coat protein gene (1000 bp) in to the 'Kirkagac 637' genome. Segregation of 4 transformants was tested by X-2 analysis and the results were consistent with predicted Mendelian ratios. In each case the P values were much greater than the rejection level of P=0.05.
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    A histological analysis of regeneration in watermelon
    (Springer India, 2003) Yalcin-Mendi, NY; Ipek, M; Kacan, H; Curuk, S; Sari, N; Cetiner, S; Gaba, V
    The optimization of regeneration protocol for different genotypes increases the yield in transformation studies. Cotyledon explants of watermelon [Citrullus lanatus (Thunb) Matsum & Nakai] cv Crimson Sweet were cultured on MS medium containing combinations of benzyl adenine (BA) (0, 5, 10, 20 muM) and indole-3-acetic acid (IAA) (0, 0.5, 5 muM). Maximum shoot growth and subsequent rooting from explants on regeneration medium were obtained from the media containing 10 muM BA + 0.5 muM IAA and 20 muM BA (75 and 78%) by direct organogenesis, respectively. Histological analysis showed that cell division was observed in the epidermal and subepidermal layers. Protuberant structures were observed in tissues between 7 and 12 days in culture. Meristematic structures were observed after 12 days in culture which later developed into buds.