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    The effect of mycorrhiza in nutrient uptake and biomass of cherry rootstocks during acclimatization
    (Ars Docendi, 2010) Aka-Kacar, Yildiz; Akpinar, Cagdas; Agar, Aslihan; Yalcin-Mendi, Yesim; Serce, Sedat; Ortas, Ibrahim
    The effect of arbuscular mycorrhizal fungi (AMF) on growth and nutrient uptake of micropropagated cherry rootstocks was evaluated during acclimatization and plant establishment. Two commonly used cherry rootstocks, 'Edabriz' and 'Gisela 5', were propagated through tissue culture and grown in a greenhouse for 16 weeks. Plantlets were inoculated with Glomus Glomus caledonium, Glomus etunicatum. Glomus intraradices, Glomus mosseae, cocktail (mixture of these species) and indigenous mycorrhiza into three different substrate mixtures. All micropropagated cherry plantlets survived transplanting. After 16 weeks. mycorrhizal plantlets had greater nutrient uptake than non-mycorrhizal plantlets. Roots of inoculated cherry plantlets were heavily colonized with AMF. These results indicated that mycorrhizal inoculation during transplantation from in vitro to ex vitro culture can induce growth responses. The experiments also showed that the mycorrhizal cherry rootstocks were healthier and had higher Zn and P contents when compared to controls for both rootstocks. G. mosseae was one of the most efficient AMF species. Indigenous AMF isolated from Cukurova region also significantly increased the plant growth and nutrient uptake. 'Gisela 5' rootstocks had significantly higher P and Zn contents than 'Edabriz'. Taken together, our results indicate that AMF inoculations enhance growth and development of micropropagated plants which would be beneficial to improve cherry rootstock production.
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    The Effects of Different Hormones on Regeneration of Gazania (Gazania rigens)
    (Ars Docendi, 2009) Yalcin-Mendi, Yesim; Unek, Ceren; Eldogan, Selay; Aka-Kacar, Yildiz; Serce, Sedat; Curuk, Pembe; Kocaman, Esra
    Recent advances in the modern techniques of propagation could help growers to meet the demand of the ornamental industry. New ornamental plant varieties are being created by breeders in response to consumer demand for new products. In this study, different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l) and IAA (0.0, 0.5, 1.0 mg/l) were investigated to optimize regeneration of Gazania rigens. The best callus formation was found from the media containing 0.2 mg/l BA-1.0 mg/l IAA (100%), 0.5 mg/l BA-1.0 mg/l IAA (98%), 0.5 mg/l BA-0.5 mg/l IAA (97%), 1 mg/l BA -1 mg/l IAA (97%), 1.0 mg/l BA-0.5 mg/l IAA (98%). 0.5 mg/l BA (97%). The most promising regeneration and growth were obtained from the media with 0.2 mg/l BA-1.0 mg/l IAA (30%). 1.0 mg/l BA-0.5 mg/l IAA (22%) and 1.0 mg/l BA (18%). The regenerated plantlets were rooted on the media containing 0.5 and 1.0 mg/l IAA. The best rooting percentage was observed on the media containing 1.0 mg/l IAA (75%).
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    Food-grade sugar can promote differentiation in melon (Cucumis melo L.) tissue culture
    (Springer, 2012) Curuk, Sebahattin; Cetiner, Selim; Yalcin-Mendi, Yesim; Carmeli-Weissberg, Mira; Graber, Ellen; Gaba, Victor
    The objective of the present study was to investigate the origin of discrepancy between experimental results in in vitro culture of Turkish melon (Cucumis melo L.) cultivars, conducted by the same individual using the same protocol and same seed batches in two different laboratories. The difference in the sucrose source was found to be the major reason for the deviation in results between the two laboratories. The percentage of regenerating explants and the number of bud-like protuberances and/or shoots were significantly greater when a food-grade Turkish sucrose was used in the medium compared with analytical-grade sucrose. Media formulated with the food-grade sucrose regenerated 37 and 67 % more explants and bud-like protuberances and/or shoots per explant, respectively, than media containing analytical-grade sucrose. No meaningful differences were found in added elements or anions between the sucrose sources or by liquid chromatography/mass spectroscopy. The only significant chemical difference observed between the sucrose samples was the presence of melanoidins (Maillard reaction products) in the food-grade sucrose. The melanoidins were of high molecular weight (> 3,000 Da determined by ultrafiltration), with characteristic ultraviolet-visible spectra and in vitro antioxidant activity. Melanoidin-containing sucrose can be differentiated by color and spectroscopy.