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Öğe Association between plant and fruit characteristics in dihaploid cantaloupe melon (Cucumis melo var cantaloupensis)(2004) Yetisir, H.; Sari, N.; Ekbiç, I.E.An experiment was conducted during the spring seasons of 1999 and 2000 to study correlations between early-appearing characteristics such as cotyledon width, length, index, seed weight and agronomic performance characteristics such as fruit diameter, length, index, and weight of 31 dihaploid cantaloupe melon (Cucumis melo L. var cantaloupensis Naudin) and also correlation between cotyledon index and fruit index. A significant positive correlation was observed between cotyledon length, and fruit length, fruit weight and 100-mature seed weight with fruit weight. A relatively lower correlation was determined between cotyledon width, and fruit diameter and fruit weight. The cotyledon index was positively correlated with fruit index. There was a low positive correlation between hypocotyle length and internode length, while hypocotyle length was positively correlated with plant length.Öğe Collection and morphological characterization of Lagenaria siceraria germplasm from the Mediterranean region of Turkey(Springer, 2008) Yetisir, H.; Sakar, M.; Serce, S.The landraces of Lagenaria siceraria in Turkey show great diversity for morphologic traits, particularly in fruit size and shape even though Turkey is not centre of genetic diversity for L. siceraria. In Turkey, L. siceraria is used as food, musical instruments and containers, according to the type and shape of their fruits. Its diversity has been gradually declining over the last 25 years. With the aim of assessing variations in plant, fruit and seed morphology among the L. siceraria landraces, 15 field trips for collection of germplasm to southern parts of Turkey (Mediterranean region) were carried out in the period of 2003-2004. The study also aimed at developing a representative core collection of the material to guide future studies and uses concerning its existing genetic diversity in Turkey. A total of 182 accessions (fruits and/or seeds) were collected. The seeds from all the sample material were sown in green house conditions at the experimental station of the Faculty of Agriculture of the Mustafa Kemal University in Hatay (Antakya), (36A degrees 18' 22'' N, 36A degrees 13' 33'' E, 82 m). In the spring of 2004, 162 out of the 182 sown seeds germinated and developed into viable seedlings. These were further transplanted in the field in 10 replicates. The morpho-agronomic characterization was carried out following the international standards for crop descriptors set by Bioversity International. The data were subject to both descriptive statistics and multivariate analysis by Principle Component Analysis. The descriptive statistics revealed that the whole collection exhibits a great deal of morphological diversity and that the subset core collection represents most of the variability. The first three principle components, calculated by using six quantitative traits, explained 26%, 21% and 17% (a total of 64%) of the total variation. Among the studied accessions, no apparently distinct patterns such as geographical origin were detected. This may suggest that the accessions have been introduced to Turkey from multiple locations and/or their diversity had been distributed almost evenly across the Mediterranean region of this country. Based on our results from the morphological characterization, 30 genotypes were selected to develop a subgroup (core) collection in order to represent most of the genetic diversity of all accessions. The work presented here is the first report about morphological characterization of Turkish L. siceraria germplasm.Öğe The improvement of resistance to bacterial speck in transgenic tomato plants by Agrobacterium tumefaciens mediated transformation(Maik Nauka/Interperiodica/Springer, 2007) Koc, N. K.; Kayim, M.; Yetisir, H.; Sari, N.; Yuceer, S. Unlu; Arici, S. E.The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to tomato genotype Urfa-2 by the LBA4404 strain of A. tumefaciens harboring the plasmid pPTC8. The presence of nptII and pto genes in transgenic plants was proved by PCR analysis. Insertion of the pto gene into the Genome of transgenic plants and expression of the gene were confirmed by southern and northern hybridizations, respectively. The pathogen P. syringae pv. tomato was applied to all leaves of transgenic and control plants. While typical bacterial speck symptoms developed on the leaves of control plants, the transgenic plants did not display any typical symptoms of bacterial speck upon inoculation with strains 1 and 0. Some of these transgenic plants had thicker leaves than the control plants and produced abnormal flowers. The pollen of transgenic plants was used for crossing with control plants to produce F1 transgenic lines. Fruits from crossed transgenic and control plants were obtained, and F1 seeds germinated on Murashige and Skoog medium in the presence of kanamycin have developed F1 seedlings.Öğe New Fusarium wilt resistant melon (Cucumis melo var. cantalupensis) varieties developed by dihaploidization: Sari F1, Yetisir F 1, Solmaz F1, Emin F1 and Yucel F1(International Society for Horticultural Science, 2010) Sari, N.; Solmaz, I.; Ekiz, H.; Yetisir, H.; Yucel, S.Researches on Galia type melon breeding was started in 1995 at Cukurova University, Turkey. The aim of this study was to develop new melon cultivars which belong to Cucumis melo var. cantalupensis group having high yield and quality, suitable for both greenhouse and open field cultivation and also resistant to race 0 and 1 of Fusarium oxysporum f. sp. melonis wilt which are common in Turkey. For this purpose 9 female parental lines were crossed with the donor male parents which are resistant to 0 and 1 races of Fusarium wilt. By this way F1 hybrids were produced, and after the first backcrosses were done, BC1 genotypes were developed. Haploid embryo inductions and haploid plant productions were obtained by pollination with irradiated pollen. These haploid plants were duplicated by colchisine treatment and dihaploid pure lines were produced. General combination ability and special combination ability tests were conducted and more than 200 F1 hybrids were developed. These hybrids were tested in open field and greenhouse conditions for many years and Sari F1 was registered in 2008, Yetisir F1, Solmaz F 1, Emin F1 and Yucel F1 were registered in 2009 by Central Certification and Registration Management of Turkish Ministry of Agriculture. These 5 varieties have some differences concerning their total and early yields, fruit size and TSSC.Öğe New Fusarium Wilt Resistant Melon (Cucumis melo var. cantalupensis) Varieties Developed by Dihaploidization: Sari F1, Yetisir F1, Solmaz F1, Emin F1 and Yucel F1(Int Soc Horticultural Science, 2010) Sari, N.; Solmaz, I.; Yetisir, H.; Ekiz, H.; Yucel, S.Researches on Galia type melon breeding was started in 1995 at Cukurova University, Turkey. The aim of this study was to develop new melon cultivars which belong to Cucumis melo var. cantalupensis group having high yield and quality, suitable for both greenhouse and open field cultivation and also resistant to race 0 and 1 of Fusarium oxysporum f. sp. melonis wilt which are common in Turkey. For this purpose 9 female parental lines were crossed with the donor male parents which are resistant to 0 and 1 races of Fusarium wilt. By this way F-1 hybrids were produced, and after the first backcrosses were done, BC1 genotypes were developed. Haploid embryo inductions and haploid plant productions were obtained by pollination with irradiated pollen. These haploid plants were duplicated by colchisine treatment and dihaploid pure lines were produced. General combination ability and special combination ability tests were conducted and more than 200 F-1 hybrids were developed. These hybrids were tested in open field and greenhouse conditions for many years and Sari F-1 was registered in 2008, Yetisir F-1, Solmaz F-1, Emin F-1 and Yucel F-1 were registered in 2009 by Central Certification and Registration Management of Turkish Ministry of Agriculture. These 5 varieties have some differences concerning their total and early yields, fruit size and TSSC.Öğe Watermelon genetic resources in Turkey and their characteristics(Int Soc Horticultural Science, 2007) Sari, N.; Solmaz, I.; Yetisir, H.; Unlu, H.Turkey is the second largest watermelon producing country after China. Although Turkey is not the origin of watermelon, it has valuable watermelon genetic resources which mainly consist of local genotypes. University of Cukurova has one of the largest collections of the country and has been studying the genetic collections since early 1990's. Today University of Cukurova has a collection of approximately 250 genetic materials. In this study, 15 provinces in 5 different regions (Southeastern Anatolia, Aegean, Marmara-Thrace, Middle Anatolia and Mediterranean regions) of Turkey were visited in summer and fall season of 2004. 134 watermelon genotypes were collected. Fruit (size, shape, ground color of skin, intensity of color, presence of stripes, flesh color) and seed (size, ground color of testa, secondary color of testa, patches at hilum, and patches at margin) of collected watermelon genotypes were characterized.