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    Pathological and Microbiological Investigations of Pneumonic Pasteurellosis in Sheep
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Ozyildiz, Zafer; Tel, Osman Yasar; Yilmaz, Rahsan; Ozsoy, Sule Yurdagul; Keskin, Oktay
    In this study, between March 2010 and March 2011, 110 pneumonia suspected lung tissues were examined histopathologically, immunohistochemically and microbiologically, in Sanliurfa province. After definition of the macroscopic localization of the consolidated areas in the lungs, tissue samples were taken and preserved in cold chain and 10% formalin for microbiological and pathological examinations, respectively. For bacteriological examination of Pasteurella spp. lung specimens were inoculated in 7% sheep blood agar and Mc Conkey agar. After routine pathological tissue follow up procedure, tissues were embedded in paraffin and obtained sections stained with Hematoxylin&Eosin (H&E). The cases, having histopathological findings consistent with pneumonia, were forwarded to immunohistochemical (IHC) examinations to know whether lesions related to Mannheimia haemolytica and Pasteurella multocida using hyperimmune polyclonal rabbit sera in Avidin Biotin Complex Peroxidase (ABC-P). Microbiological, histopathological and immunohistochemical findings were comparatively evaluated in examined animals. Pasteurella multocida as a cause of pneumonia were detected in 38 cases of microbiological inoculations. Immunohistochemical staining resulted Mannheimia haemolytica (n=35) and Pasteurella multocida (n=30) positive. Immunohistochemically both Mannheimia haemolytica and Pasteurella multocida were positive in 23 cases and 45 animals were negative for both bacteria. The aim of this study is to show importance and role of Pasteurella spp, in sheep pneumonia in Sanliurfa region.
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    The scintigraphic, biodistribution and histopathological evaluation of the effect of experimental 131I administration on the gastrointestinal system and the demonstration of the Na+/I- symporter by immunohistochemistry in rats
    (Lippincott Williams & Wilkins, 2018) Yilmaz, Rahsan; Yumusak, Nihat; Sadic, Murat; Atilgan, Hasan I.; Korkmaz, Meliha; Bozkaya, Faruk
    ObjectiveThe aim of this study was to investigate histopathological changes and biodistribution of iodine-131 (I-131) in the gastrointestinal system (GIS) and also Na+/I- Symporter (NIS) presence by immunohistochemically in the experimental treatment of rats with radioactive iodine (RAI).Materials and methodsRats were divided into experimental and control groups as random early group 2 (24h), intermediate group 3 (3 weeks), and late period group 4 (3 months). Experimental groups were administered 100MBq (approximate to 3mCi, 12mCi/kg) by orogastric route with orogastric tube. Scintigraphic iodine screening images were obtained 24h, 3 weeks, and 3 months after RAI, and GIS tissues were removed, and immunohistochemical methods were used to demonstrate NIS with RAI biodistribution and histopathology.ResultsAccording to the results of scintigraphy, the most prominent activity involvement was observed in the thyroid gland at group 2, and significant activity was observed in the stomach. In the group 3 and group 4 images, owing to the physiological and biological half-life of the iodine and low resolution of the gamma camera, no secondary focal activation was observed. The highest RAI biodistribution value in all groups was in the stomach, ileum and oesophagus. In the immunohistochemical examination of NIS, the highest staining sequence was observed in all groups respectively in the stomach, oesophagus, tongue, colon, saliva, duodenum, rectum, ileum and jejunum. The increase of NIS immunohistochemically stained more intensely was observed in the RAI-administered groups.ConclusionThe amount of NIS is important for the absorption of RAI after administration.