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    Öğe
    45S5 Bioactive Glass-Ointment Positively Effects on Wound Healing in Rats by Regulating TNF?, Il-10, VEGF, and TGF?
    (Wiley, 2024) Kirgiz, Omer; Altug, Muhammed Enes; Ozkan, Huseyin; Han, Mehmet Cengiz; Akcakavak, Goekhan; Ozarslan, Ali Can; Yucel, Sevil
    AimThis study aimed to investigate the effects of 45S5 bioactive glass-ointment (BG) on cutaneous wound healing in rats at the molecular, biochemical, and histopathological levels.Materials and MethodsThirty-two rats were divided into four groups (n = 8): Control, Sham, BG, and DEX (Dexpanthenol). While no wound treatment was applied to the CONTROL, a wound model was created in the Sham, and no treatment was applied. A wound model was created for other groups, and BG and DEX were applied locally for 21 days. During the 21-day experiment period, feed and water consumption and weight changes were observed. Wound areas were calculated on days 0, 3, 7, 4, and 21. Following treatment, the rats were euthanized and tissues from the wound area and blood samples were collected. While the expression levels of tumor necrosis factor-alpha (TNF alpha), Interleukin 6 (IL6), Interleukin 10 (IL10), transforming growth factor-beta (TGF beta), and vascular endothelial growth factor (VEGF) genes were determined by qPCR, the levels of TNF alpha, IL6, and IL10 proteins were measured by ELISA.ResultsIt was observed that the BG group showed anti-inflammatory activity by suppressing TNF alpha levels and stimulating IL-10. In addition, it was determined that BG increased fibroblast activity and vascularization.ConclusionCurrent findings showed that topical application of BG has anti-inflammatory effects, while also accelerating healing by increasing vascularity and making positive contributions to tissue healing. This study investigates the effects of topical application of 45S5 bioactive glass-ointment (BG) on wound healing in rats at the molecular and histopathological levels. The findings demonstrate that BG exhibits anti-inflammatory activity, enhances vascularization, accelerates healing, and contributes positively to tissue repair.image
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    Öğe
    Green Electrospun Poly(vinyl alcohol)/Gelatin-Based Nanofibrous Membrane by Incorporating 45S5 Bioglass Nanoparticles and Urea for Wound Dressing Applications: Characterization and In Vitro and In Vivo Evaluations
    (Amer Chemical Soc, 2024) Temel-Soylu, Tulay Merve; Kececiler-Emir, Ceren; Rababah, Taha; Ozel, Cem; Yucel, Sevil; Basaran-Elalmis, Yeliz; Altan, Dilan
    This study reports the fabrication and characterization of poly(vinyl alcohol) (PVA) and gelatin (Gel)-based nanofiber membranes cross-linked with citric acid (CA) by a green electrospinning method in which nano 45S5 bioglass (BG) and urea were incorporated. Various combinations of PVA, gelatin, and BG were prepared, and nanofiber membranes with average fiber diameters between 238 and 595 nm were fabricated. Morphological, chemical, and mechanical properties, porosity, swelling, water retention, and water vapor transmission rate of the fabricated membranes were evaluated. PVA:Gel (90:10), 15% CA, and 3% BG were determined as the optimum blend for nanofiber membrane fabrication via electrospinning. The membrane obtained using this blend was further functionalized with 10% w/w polymer urea coating by the electrospray method following the cross-linking. In vitro biocompatibility tests revealed that the fabricated membranes were all biocompatible except for the one that functionalized with urea. In vivo macroscopic and histopathological analysis results of PVA/Gel/BG and PVA/Gel/BG/Urea treated wounds indicated increased collagenization and vascularization and had an anti-inflammatory effect. Furthermore, careful examination of the in vivo macroscopic results of the PVA/Gel/BG/Urea membrane indicated its potential to decrease uneven scar formation. In conclusion, developed PVA/Gel/BG and PVA/Gel/BG/Urea electrospun membranes with multifunctional and biomimetic features may have the potential to be used as beneficial wound dressings.