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    Molecular Characterization of Staphylococcus aureus from Clinical Sheep Mastitis Cases
    (Israel Veterinary Medical Assoc, 2022) Aslantas, O.; Keskin, O.; Yucetepe, Gullu A.
    In the current study, it was aimed to examine the genes encoding toxin, biofilm and microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) of 62 Staphylococcus aureus isolated from sheep clinical mastitis cases using polvmerase chain reaction (PCR). Besides, accessory gene regulator (agr) groups and the antibiotic susceptibilities of the isolates were also determined. Most of the isolates (87.1%) were susceptible to all tested antimicrobials. The sec (38, 61.3%) and sell (50, 80.6%) were the only staphylococcal enterotoxin (SE) genes detected among the isolates. The tst gene was observed in 43 (57.3%) isolates. Whereas co-existence of sec, sell, tst was detected in 36 (48%) isolates; none of them isolates harbored eta and eth genes. However, eight (12.9%) isolates were found to be negative for SE, ET and TSST-1 genes. Hemolysin genes were detected in all isolates and the frequency of bla,hlb,hld, and hlg2 were 100%, 95.2%, 98.4%, and 61.3%, respectively. The lukED and lukM were found together in 40 (64.5%) of the isolates, and /ukED was found alone in 22 (35.5%) of the isolates. Of the biofilm-related genes, icaD was the only gene detected with a frequency of 83.9% (52). MSCRAMMs genes eno, cna, ebps, fib, fnb, clfA, and c()13 were detected in 100%, 79%, 100%, 95.2%, 100%, and 100%, respectively. None of the isolates carried the bbp gene. This study indicated that S. aureus isolates from clinical sheep mastitis possessed several virulence-associated determinants.