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Öğe Isolation and molecular characterization of extended spectrum beta-lactamase (ESBL) producing escherichia coli from cage birds in Adana region, Turkey(Kafkas Üniversitesi, 2018) Sakin, Fatih; Müjde, Cem; Aslantaş, ÖzkanIn this study, it was aimed to investigate the presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli in caged birds. A total of 139 fecal swab samples were collected from cage birds from 14 different aviaries in Adana. ESBL producing E. coli was detected in 4.3% (n=6) of the fecal samples and these isolates harbored blaCTX-M-15 gene. ERIC-PCR analysis revealed three different band patterns among the isolates. These results indicate that cage birds are carrier of ESBL-producing E. coli. Therefore, further epidemiological studies are needed to determine the presence of resistant bacteria including ESBL producing E. coli in various animal species.Öğe Gene expression levels in some candidate genes for mastitis resistance, milk yield, and milk quality of goats reared under different feeding systems(TÜBİTAK, 2018) Yakan, Akın; Özkan, Hüseyin; Eraslan Şakar, Aysel; Ünal, Necmettin; Özbeyaz, CeyhanThis study aimed to identify the expression levels of some candidate genes for mastitis resistance ( LT F and BRCA1 ), milk yield ( POU1F1 and IGF-1 ), and milk quality ( PPARγ and CSN2 ) in different feeding systems (pen- and pasture-based) used for Damascus goats. Milk samples were taken from all goats in both pen and pasture groups at 4 times, at the 1st, 3rd, 5th, and 7th months of the study. It was found that, compared to the pen group, the LT F and CSN2 genes in the pasture group had significant levels of upregulation in the 7th month of lactation (3.538- and 5.436-fold, respectively), the PPARγ gene had downregulation at the beginning of lactation (0.399-fold), and the IGF-1 gene had upregulation in the 3rd month of lactation (2.313-fold) but had significant levels of downregulation (2.332-fold) in the 5th month of lactation. It was determined that LT F gene expression levels in goats might be used as a criterion in identifying mastitis resistance, IGF-1 for lactation milk yield, and PPARγ for milk fat yield. This study is the first report that presents LT F , BRCA1 , IGF-1 , and PPARγ gene expression levels in goat milk and udder epithelial cells and the effect of BRCA1 , POU1F1 , and PPARγ genes on milk protein yield.Öğe Is total RNA extraction possible from small volume goat milk (7.5 mL) for RT-qPCR studies ?(Ankara Üniversitesi, 2018) Eraslan Şakar, Aysel; Özkan, Hüseyin; Yakan, AkınThis study aimed to study the application availability of RT-qPCR by total RNA extraction from small volumes of milk samples. RNA was extracted from 7,5 mL milk. Amount and quality of RNA and Ct results obtained via RT-qPCR were compared with the values obtained from 100 mL milk. A260/A280 ratio of absorbance was found to be higher than 1,70 in all samples. Mean RNA purity were identified as ,87±0,07 and 1,77±0,01 in 7,5 mL and 100 mL milk samples respectively. While mean total RNA concentration was 149,37±43,40 μg/mL in 7,5 mL milk sample, it was measured to be 309,03±77,82 μg/mL in 100 mL milk sample. Mean somatic cell count (SCC) of the 8 goats used in the study was calculated as 486,37±185,68 x 103 cells/mL. Ribosomal Protein Lateral Stalk Subunit P0 (RPLP0) gene which is accepted as the reference gene in goat somatic cells was used to test application availability of obtained RNA in RT-qPCR. Mean Ct results were found to be 20,33±0,39 and 19,51±0,41 in 7,5 mL and 100 mL groups respectively, creating no difference between the two groups (P = 0,170). Quality and amount of obtained RNA and Ct results showed that it was possible to extract RNA by using 7,5 mL milk.Öğe Unilateral uterine torsion in a pregnant bitch(Selçuk Üniversitesi Veteriner Fakültesi, 2018) Doğruer, Gökhan; Köse, Ayşe Merve; Koldaş Ürer, Ece; Doğruer, AzimeThis report describes the diagnosis and treatment of a case of unilateral uterine torsion, which was detected at the caesarean section of a pregnant bitch. A one-year-old, 34 kg half-breed Kangal dog, with signs of apathy, anorexia and emesis for the past two days was the material of this case report. The dog only displayed a mild apathy. Mucous membranes colour, capillary refilling time, heart and respiratory rates were within the physiological ranges. The body temperature was 37.5°C. A mucoid brown-greenish coloured vaginal discharge was determined. The measured biochemical values fell within the physiological ranges. Both live and dead foetuses were determined ultrasonographically then the number of dead foetuses increased half an hour later on second ultrasonographic examination so it was decided to perform a caesarean section. One female and two male live foetuses from the right uterine horn and two dead female foetuses from the left uterine horn were removed. The left uterine horn displayed hyperaemia, haemorrhage and fragility with a 1800° torsion along its long axis, near the uterine bifurcation. The amniotic fluids appeared normal, the chorioallantoic fluid was haemorrhagic in the left horn. Total ovariohysterectomy was performed. The uterine torsion cases may be manifested with nonspecific clinical signs such as anorexia and emesis. In the event of increased number of dead foetuses in repeated ultrasonographic examination, the presence of uterine torsion should be considered. In such cases, the pregnant animal can be saved by surgical intervention, as was in this case study.
