Characterization of extracellular esterase and lipase activities from five halophilic archaeal strains

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dc.contributor.authorOzcan, Birgul
dc.contributor.authorOzyilmaz, Gul
dc.contributor.authorCokmus, Cumhur
dc.contributor.authorCaliskan, Mahmut
dc.date.accessioned2024-09-18T20:26:33Z
dc.date.available2024-09-18T20:26:33Z
dc.date.issued2009
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractA total of 118 halophilic archaeal collection of strains were screened for lipolytic activity and 18 of them were found positive on Rhodamine agar plates. The selected five isolates were further characterized to determine their optimum esterase and lipase activities at various ranges of salt, temperature and pH. The esterase and lipase activities were determined by the hydrolysis of pNPB and pNPP, respectively. The maximum hydrolytic activities were found in the supernatants of the isolates grown at complex medium with 25% NaCl and 1% gum Arabic. The highest esterase activity was obtained at pH 8-8.5, temperature 60-65A degrees C and NaCl 3-4.5 M. The same parameters for the highest lipase activities were found to be pH 8, temperature 45-65A degrees C and NaCl 3.5-4 M. These results indicate the presence of salt-dependent and temperature-tolerant lipolytic enzymes from halophilic archaeal strains. Kinetic parameters were determined according to Lineweaver-Burk plot. The KM and V (max) values were lower for pNPP hydrolysis than those for pNPB hydrolysis. The results point that the isolates have higher esterase activity comparing to lipase activity.en_US
dc.description.sponsorshipMustafa Kemal University [06 F 302]; Scientific and Technical Research Council of Turkey (TUBITAK) [105T041, 107T919]en_US
dc.description.sponsorshipThis study was supported by the Research Project Units of Mustafa Kemal University (project number 06 F 302) and in part by the Scientific and Technical Research Council of Turkey (TUBITAK) (project numbers 105T041 and 107T919).en_US
dc.identifier.doi10.1007/s10295-008-0477-8
dc.identifier.endpage110en_US
dc.identifier.issn1367-5435
dc.identifier.issn1476-5535
dc.identifier.issue1en_US
dc.identifier.pmid18830729en_US
dc.identifier.scopus2-s2.0-57649210759en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage105en_US
dc.identifier.urihttps://doi.org/10.1007/s10295-008-0477-8
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10388
dc.identifier.volume36en_US
dc.identifier.wosWOS:000261751500013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofJournal of Industrial Microbiology & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHalophilic archaeaen_US
dc.subjectEsteraseen_US
dc.subjectLipaseen_US
dc.subjectCharacterizationen_US
dc.titleCharacterization of extracellular esterase and lipase activities from five halophilic archaeal strainsen_US
dc.typeArticleen_US

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