Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I-furcatus

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dc.authoridLi, Chao/0000-0002-1950-0190
dc.authoridLipke, Elizabeth/0000-0002-3465-5609
dc.contributor.authorShang, Mei
dc.contributor.authorSu, Baofeng
dc.contributor.authorLipke, Elizabeth A.
dc.contributor.authorPerera, Dayan A.
dc.contributor.authorLi, Chao
dc.contributor.authorQin, Zhenkui
dc.contributor.authorLi, Yun
dc.date.accessioned2024-09-18T20:08:05Z
dc.date.available2024-09-18T20:08:05Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractTesticular germ cells of channel catfish, Ictalurus punctatus, and blue catfish, I. furcatus were separated into four layers with Percoll density gradient centrifugation, containing different cell types (40 % in the first layer were spermatogonial stem cells, SSCs). Expression of seventeen genes was analyzed for cells from different layers by real-time quantitative PCR. Pfkfb4, Urod, Plzf, Integrin6, IntegrinV, Thy1 and Cdh1 genes showed the same expression change pattern in both channel and blue catfish as these genes were down-regulated in the spermatocytes and even more so in spermatids. Plzf and Integrin6 had especially high expression in SSCs and can be used as SSCs specific markers. Sox2 gene was up-regulated in spermatocytes and even more highly up-regulated in spermatids, which indicated it could be a spermatid marker. In contrast to channel catfish, Id4, Smad5 and Prdm14 gene expressions were strongly down-regulated in spermatocyte cells, but up-regulated in spermatid cells in blue catfish. Smad5 gene was down-regulated in spermatocytes, but up-regulated in both spermatogonia and spermatids, allowing identification as a marker for spermatocytes in blue catfish. Oct4, Id4, Gfr alpha 2, Pum2 and Prdm14 genes showed different expression patterns in the testicular germ cells of channel and blue catfish. This may be a partial explanation to the differing responses of channel catfish and blue catfish to induced spawning technologies. The SSCs specific markers can be used for further SSCs labeling, which can increase the SSCs sorting efficiency and be applied in various studies involving SSCs and other germ cells.en_US
dc.description.sponsorshipUSDA-Biotechnology Risk Assessment Program [2009-33522-05774]; Alabama Agricultural Experiment Station [80-009]; Office of the Vice President for Research, Auburn University (OVPR-AU-IGP); NIFA [581528, 2009-33522-05774] Funding Source: Federal RePORTERen_US
dc.description.sponsorshipThis work was partially funded by the USDA-Biotechnology Risk Assessment Program (Grant No. 2009-33522-05774), the Alabama Agricultural Experiment Station (80-009) and the Office of the Vice President for Research, Auburn University (OVPR-AU-IGP).en_US
dc.identifier.doi10.1007/s10695-015-0106-1
dc.identifier.endpage1556en_US
dc.identifier.issn0920-1742
dc.identifier.issn1573-5168
dc.identifier.issue6en_US
dc.identifier.pmid26251285en_US
dc.identifier.scopus2-s2.0-84947027640en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1545en_US
dc.identifier.urihttps://doi.org/10.1007/s10695-015-0106-1
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8622
dc.identifier.volume41en_US
dc.identifier.wosWOS:000364517600016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofFish Physiology and Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCatfishen_US
dc.subjectSpermatogonia stem cellen_US
dc.subjectDensity gradient centrifugationen_US
dc.subjectGene expressionen_US
dc.subjectMarker geneen_US
dc.titleSpermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I-furcatusen_US
dc.typeArticleen_US

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