Effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (Cyprinus carpio) sperm

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorYildiz, Cengiz
dc.contributor.authorYavas, Ilker
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorAksoy, Melih
dc.date.accessioned2024-09-18T20:13:34Z
dc.date.available2024-09-18T20:13:34Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAddition of cholesterol-loaded cyclodextrin (CLC) to the diluents of mammalian semen increased stability and rigidity of phospholipid hydrocarbon chains of plasma membrane during sperm cryopreservation process. CLC has been tested successfully as ayoprotectant in various livestock sperm cryopreservation protocols but its efficacy for cryopreserving of fish sperm has not previously been tested. In the present study, different cholesterol loaded cyclodextrin concentrations were evaluated for the cryopreservation of carp (Cyprinus carpio) sperm. Sexually mature fish were induced to spermiation and ovulation with Ovopel. The extenders were prepared by using 300 mM glucose and 10% DMSO supplemented with different concentrations of CLC (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mg per 120 x 10(6) spermatozoa) and without CLC (control). The pooled semen was diluted separately at a ratio of 1:3 (v/v) by using CLC extenders. Diluted semen placed into 0.25 ml straws were equilibrated at 4 degrees C for 15 min and frozen in liquid nitrogen vapor. Fertilization was conducted using a ratio of 1 x 105 spermatozoa/egg. Fresh sperm with no treatment showed the greatest sperm motility, duration of motility, viability, and fertilization results compared to the other tested cryopreserved and control groups (p < 0.05). Supplementation of 1.5 mg CLC to the extender showed the best cryoprotective effect for sperm motility, duration of motility, and viability against freezing damage in comparison to extenders containing 2.5 mg, 3.0 mg CLC, and control group (p < 0.05). Cryopreserved sperm containing 1.5 mg CLC provided greater result in term of fertilization success when compared to other extenders containing 0.5, 2.5, and 3.0 mg CLC or control (p <0.05). The amount of CLC effected post-thaw sperm quality and fertility as a dose-dependent manner. It is concluded that treatment of cholesterol-loaded cyclodextrin for carp sperm cryopreservation significantly improves cell cryosurvival and fertilization. (C) 2015 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific Research Institute of Mustafa Kemal University [MKU-08-E-0203]en_US
dc.description.sponsorshipThis study was funded by a grant from Scientific Research Institute of Mustafa Kemal University (MKU-08-E-0203).en_US
dc.identifier.doi10.1016/j.cryobiol.2015.01.009
dc.identifier.endpage194en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue2en_US
dc.identifier.pmid25661710en_US
dc.identifier.scopus2-s2.0-84925163882en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage190en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2015.01.009
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9266
dc.identifier.volume70en_US
dc.identifier.wosWOS:000352673900016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryopreservationen_US
dc.subjectCholesterolen_US
dc.subjectCyclodextrinen_US
dc.subjectCarpen_US
dc.subjectFreezingen_US
dc.subjectSpermen_US
dc.subjectFertilizationen_US
dc.titleEffect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (Cyprinus carpio) spermen_US
dc.typeArticleen_US

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