Genotoxicity of a synthetic plant growth regulator, Forchlorfenuron (CPPU), on human lymphocytes using chromosome aberration assay

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dc.authorscopusid15081246100
dc.authorscopusid58621751000
dc.contributor.authorKocaman, Ayşe Yavuz
dc.contributor.authorYakar, Berna
dc.date.accessioned2024-09-19T15:45:31Z
dc.date.available2024-09-19T15:45:31Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractForchlorfenuron (FCF, also known as CPPU), which belongs to the group of phenylurea cytokinins, is one of the most widely used synthetic plant growth regulators (PGRs) worldwide. Although FCF plays a crucial role in cellular growth and differentiation by promoting cell division in plants, it disrupts higher-order septin assembly in other eukaryotic organisms, including humans. Despite its widespread use, no study has been found investigating the genotoxic effects of this synthetic PGR on humans. Hence, this investigation was designed to examine the potential cyto-genotoxicity of a commercial formulation of FCF on human peripheral blood lymphocytes (PBLs) using chromosome aberrations (CAs) and mitotic index (MI) endpoints. The whole blood cultures were treated with 0.25, 0.50, 1.00, and 2.00 µg/ml concentrations of a commercial form of FCF. According to the results, FCF significantly enhanced the percentage of cells containing structural CAs at the concentrations of 1.00 and 2.00 µg/ml for both treatment times (24 and 48 h), in comparison to the negative control (P<0.05). Besides, in cultures exposed to FCF concentrations of 0.50, 1.00, and 2.00 µg/ml, the total CA/ cell ratio was significantly higher (P<0.05). In addition, FCF was found to have cyto-toxic activity on human PBLs at all treatments (except for the lowest concentration at 24 h) by significantly reducing the MI compared to the negative control (P<0.05). The findings of this investigation indicate the first time that a commercial formulation of FCF (0.50-2.00 µg/ml) may have genotoxic and cytotoxic potential on human lympho-cytes. © 2023 Ayşe Yavuz Kocaman,Berna Yakar.en_US
dc.identifier.doi10.36253/caryologia-2006
dc.identifier.endpage56en_US
dc.identifier.issn0008-7114
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85172444775en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage47en_US
dc.identifier.urihttps://doi.org/10.36253/caryologia-2006
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14748
dc.identifier.volume76en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFirenze University Pressen_US
dc.relation.ispartofCaryologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectchromosome aberrationen_US
dc.subjectCPPUen_US
dc.subjectForchlorfenuronen_US
dc.subjecthuman lymphocytesen_US
dc.subjectmitotic indexen_US
dc.subjectsynthetic plant growth regulatoren_US
dc.titleGenotoxicity of a synthetic plant growth regulator, Forchlorfenuron (CPPU), on human lymphocytes using chromosome aberration assayen_US
dc.typeArticleen_US

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