Effect of different avian egg yolk types on fertilization ability of cryopreserved common carp (Cyprinus carpio) spermatozoa

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.contributor.authorYildiz, Cengiz
dc.date.accessioned2024-09-18T20:14:58Z
dc.date.available2024-09-18T20:14:58Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn spite of the fact that egg yolk from different avian species has successfully been used as an additive for the cryopreservation of sperm in mammalian species, its efficacy for cryopreserving fish sperm has not previously been tested comparatively. Therefore, the present study was carried out to determine the effect of egg yolks from different avian species, namely domestic chicken (Gallus gallus domesticus), turkey (Meleagris gallopavo) and quail (Coturnix coturnix), on post-thaw motility and fertilization ability of cryopreserved common carp spermatozoa. Egg yolks from chicken, turkey and quail were analysed for moisture, total fat, protein, cholesterol and phospholipid profile. Total fat and cholesterol contents of the turkey egg yolk were higher than chicken and quail egg yolks (p < 0.05). Semen was frozen according to conventional slow freezing procedure. The extender contained 350 mM glucose, 30 mM Tris and 5 % glycerol supplemented with different ratios of avian egg yolk (10, 15 and 20 %). Semen was equilibrated at 4 A degrees C for 15 min and placed into 0.25-ml straws and frozen in liquid nitrogen vapour (for 10 min at -120 A degrees C) and finally stored in liquid nitrogen (-196 A degrees C) tank. The frozen spermatozoa were thawed in a water bath at 35 A degrees C for 30 s. Fertilization was conducted using a ratio of 1 x 10(5) spermatozoa/egg. Cryopreservation experiments resulted in higher post-thaw motility and fertilization rates. Mean post-thaw motility of cryopreserved spermatozoa was between 45 and 80 %, and fertilization rates, expressed as the percentage of eyed embryos, ranged from 70 to 95 %. In conclusion, the present study showed that turkey and quail egg yolks are suitable alternatives to the chicken egg yolk for the cryopreservation of common carp spermatozoa.en_US
dc.description.sponsorshipScientific Research Institute of Mustafa Kemal University [MKU-08-E-0203]en_US
dc.description.sponsorshipThis research was funded by a Grant from Scientific Research Institute of Mustafa Kemal University (MKU-08-E-0203). The authors would like to thank the staff of the State Hydraulic Works (SHW) Fish Production Station in Adana (Turkey) for their technical assistance. Also, the valuable comments and suggestions from anonymous reviewers are deeply thanked.en_US
dc.identifier.doi10.1007/s10499-013-9728-4
dc.identifier.endpage139en_US
dc.identifier.issn0967-6120
dc.identifier.issn1573-143X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84892445261en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage131en_US
dc.identifier.urihttps://doi.org/10.1007/s10499-013-9728-4
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9345
dc.identifier.volume22en_US
dc.identifier.wosWOS:000329939600013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAquaculture Internationalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCyprinus carpioen_US
dc.subjectCryopreservationen_US
dc.subjectEgg yolken_US
dc.subjectSperm qualityen_US
dc.subjectFertilizationen_US
dc.titleEffect of different avian egg yolk types on fertilization ability of cryopreserved common carp (Cyprinus carpio) spermatozoaen_US
dc.typeArticleen_US

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