Cryopreservation of Nile Tilapia (Oreochromis niloticus) Sperm

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.coverage.doi10.5772/62605
dc.date.accessioned2024-09-18T20:52:55Z
dc.date.available2024-09-18T20:52:55Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractThe main aim of this study is to determine the effect of the straw volume (0.25 vs. 0.5 mL) on Nile tilapia sperm quality after cryopreservation. Sperm was frozen according to conventional slow freezing procedure and diluted at ratio of 1: 3 with ionic extender containing 350 mM glucose and 30 mM Tris containing 10% dimethylacetamide. Diluted semen was equilibrated at 4 degrees C for 10 min and drawn into 0.25-mL or 0.5-mL plastic straws and sealed with polyvinyl alcohol. Samples were frozen 3 cm above of the liquid nitrogen surface and exposed to the liquid nitrogen vapor (approximate to-140 degrees C) for 10 min. After this, frozen sperm cells were kept into the liquid nitrogen container (-196 degrees C). The frozen sperm in different volume of straws were thawed in a water bath at 30 degrees C for 20 s (0.25-mL straws) or at 30 degrees C for 30 s (0.5-mL straws), respectively. Fertilization was conducted using 1 x 10(5) spermatozoa/egg ratio with each straw type. The findings of the present study indicated that cryopreservation of sperm in glucose-Tris-based extender using 0.5-mL straws improved post-thaw progressive motility, duration of progressive motility, and fertilization results (P<0.01). On the other hand, differences in term of post-thaw cell viability was not significant among the treatments (P>0.01). In conclusion, our results suggest that Nile tilapia sperm can be successfully cryopreserved in Tris-based extenders supplemented with glucose containing 10% dimethylacetamide in 0.5-mL straws.en_US
dc.identifier.doi10.5772/64835
dc.identifier.endpage90en_US
dc.identifier.isbn978-953-51-2780-2
dc.identifier.isbn978-953-51-2779-6
dc.identifier.startpage75en_US
dc.identifier.urihttps://doi.org/10.5772/64835
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11490
dc.identifier.wosWOS:000399351500006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherIntech Europeen_US
dc.relation.ispartofCryopreservation in Eukaryotesen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOreochromis niloticusen_US
dc.subjectspermen_US
dc.subjectcryopreservationen_US
dc.subjectstraw volumeen_US
dc.subjectdimethylacetamideen_US
dc.titleCryopreservation of Nile Tilapia (Oreochromis niloticus) Spermen_US
dc.typeBook Chapteren_US

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