Detection and characterization of Plum Pox Potyvirus (PPV) by DAS-ELISA and RT-PCR / RFLP analysis in Turkey

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dc.authorscopusid56619351300
dc.authorscopusid14038176800
dc.authorscopusid55945299100
dc.contributor.authorSertkaya, Gülşen
dc.contributor.authorUlubaş, Çi?dem
dc.contributor.authorÇa?layan, Kadriye
dc.date.accessioned2024-09-19T15:47:10Z
dc.date.available2024-09-19T15:47:10Z
dc.date.issued2003
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study was conducted to determine the presence of plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterize PPV isolates by serelogical and molecular techniques, including ELISA and PCR/RFLP. Thus, leaf samples from different stone fruit species (almond, apricot, nectarine, peach, plum and sour and sweet cherry) exhibiting various types of symptoms related to PPV were collected from different parts of the canopy from randomly selected orchards in the main stone fruit growing areas in Turkey, except for Malatya province. Polyclonal antibodies were used to detect the presence of PPV in the plant samples by serological assays (DAS-ELISA). The following monoclonal antibodies (MAbs): Mab 5B (Universal), Mab 4DG5 (PPV-D: Dideron-specific), Mab AL (PPV-M: Marcus-specific). Mab EA24 (PPV-El Amar-specific) and Mab AC (PPV-C: Cherry-specific), were used to identify the serotyping of PPV isolates. Reverse transcription-polymerase chain reaction (RT-PCR) assays and restriction fragment length polymorphism analysis of RT-PCR products were performed to characterize Turkish PPV isolates. The results of RT-PCR analyses using general primers were in complete agreement with the DAS-ELISA and DASI-ELISA results, showing that 2 of 52 stone fruit samples collected from apricots in Ankara province were infected with the M strain of PPV. This study confirmed the results of the previous work and demonstrated the presence of the PPV-M strain in apricots in Turkey.en_US
dc.identifier.doi10.3906/tar-0301-8
dc.identifier.endpage220en_US
dc.identifier.issn1300-011X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0242540012en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage213en_US
dc.identifier.urihttps://doi.org/10.3906/tar-0301-8
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15024
dc.identifier.volume27en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherTUBITAKen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme-linked immunosorbent assayen_US
dc.subjectPlum pox potyvirusen_US
dc.subjectRestriction analysisen_US
dc.subjectReverse transcription-polymerase chain reactionen_US
dc.subjectSharkaen_US
dc.titleDetection and characterization of Plum Pox Potyvirus (PPV) by DAS-ELISA and RT-PCR / RFLP analysis in Turkeyen_US
dc.title.alternativeTürkiye'de Plum Pox (Şarka) Virüsünün DAS-ELISA ve RT-PCR/RFLP Analizi ile Saptanmasi ve Tanilanmasien_US
dc.typeArticleen_US

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