A novel application for determination of germin gene products in situ

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dc.authorscopusid7005185601
dc.authorscopusid6603825851
dc.authorscopusid6701491865
dc.contributor.authorÇalişkan, M.
dc.contributor.authorOzcan, B.
dc.contributor.authorCuming, A.C.
dc.date.accessioned2024-09-19T15:48:47Z
dc.date.available2024-09-19T15:48:47Z
dc.date.issued2003
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn situ nucleic acid hybridization is one of the most powerful techniques developed for localizing the expression site of a particular gene at the cell, tissue and organ levels. This method is especially useful in understanding the function of specific gene products in particular tissues and the relation between tissue function and its localization in the whole structure of an organ. In this system, labeled anti-sense RNA probes were used to hybridize with desired mRNA while labeled sense probes were used as negative control. In general, each of these labeled probes applied to successive sections rather then the same section. That might cause some uncertainty if there are any spatial differences in the expression of gene products. In the current study, in our knowledge for the first time, anti-sense and sense probes were applied to the same sample to overcome any spatial differentiation of gene expression. The technique was successfully used to localize a plant gene product by applying anti-sense probes to the one site of the section while the other site of the same section was reacted with sense probes for control hybridization. © 2003 Taylor and Francis Group, LLC.en_US
dc.identifier.doi10.1080/13102818.2003.10817053
dc.identifier.endpage26en_US
dc.identifier.issn1310-2818
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-1242329338en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage22en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2003.10817053
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15306
dc.identifier.volume17en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology and Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTriticum aestivumen_US
dc.subjectcomplementary RNAen_US
dc.subjectgene producten_US
dc.subjectmessenger RNAen_US
dc.subjectarticleen_US
dc.subjectcontrolled studyen_US
dc.subjectgene expressionen_US
dc.subjectgene locationen_US
dc.subjectgenetic analysisen_US
dc.subjectin situ hybridizationen_US
dc.subjectmolecular probeen_US
dc.subjectnonhumanen_US
dc.subjectRNA hybridizationen_US
dc.subjectRNA probeen_US
dc.subjectspring wheaten_US
dc.subjecttechniqueen_US
dc.titleA novel application for determination of germin gene products in situen_US
dc.typeArticleen_US

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