Proapoptotic Effect of Hypericum perforatum (St. John's Wort) Extract in Human Colorectal Adenocarcinoma Cell Line HT29

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dc.authoridTekeli, Ibrahim Ozan/0000-0002-6845-2279
dc.contributor.authorYegani, Arash A.
dc.contributor.authorIstifli, Erman S.
dc.contributor.authorTekeli, Ibrahim O.
dc.contributor.authorSakin, Fatih
dc.contributor.authorKaplan, Halil M.
dc.date.accessioned2024-09-18T20:26:32Z
dc.date.available2024-09-18T20:26:32Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractBackground and Objective: Hypericum perforatum (HP) is known to have therapeutic effects in many types of cancer. Although the active substances hypericin and hyperforin in the composition of HP have been shown to exert apoptotic effects in cancer cell lines, research into the anticancer effects of the total extract of HP is meagre. The aim of this study was to investigate the proapoptotic effect of HP on human colorectal adenocarcinoma cell line HT29. Materials and Methods: HT29 cells (5 X 10(4) cells cm(-2)) were exposed to 99.2% medium and pharmacologically effective concentration of HP extract (0.8%) for 48 h. Cells were then washed in phosphate buffered saline PBS and lysed in RIPA buffer for 15 min on ice followed by centrifugation at 15000 rpm for 20 min and supernatants were taken and pellets were discarded. ELISA test was used to examine the expression and activity of cleaved caspase-3, Bax, Bcl-2, Wee1, GADD153, GRP78 and AIF proteins in HT29 cells. Results: ELISA test results indicated that the activities of proapoptotic proteins Bax, cleaved-caspase 3, Wee1, GRP78, GADD153 and AIF in human HT29 cell line were significantly increased in 48 h treatment with HP extract at 0.8% concentration. On the other hand, the activity of Bcl-2 was observed to decrease significantly. Conclusion:The results implicated that the HP extract in its original form effectively induces proapoptotic alterations in HT29 cells and a possible mutual signaling between the proapoptotic Wee1, GRP78, GADD153, AIF, Bax and cleaved caspase-3 proteins can contribute to this effect.en_US
dc.description.sponsorshipMustafa Kemal University Research Projects Unit [16881]en_US
dc.description.sponsorshipThis study was funded by Mustafa Kemal University Research Projects Unit (Project No.: 16881).en_US
dc.identifier.doi10.3923/ijp.2020.120.125
dc.identifier.endpage125en_US
dc.identifier.issn1811-7775
dc.identifier.issn1812-5700
dc.identifier.issue2en_US
dc.identifier.startpage120en_US
dc.identifier.urihttps://doi.org/10.3923/ijp.2020.120.125
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10376
dc.identifier.volume16en_US
dc.identifier.wosWOS:000526081000005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAsian Network Scientific Information-Ansineten_US
dc.relation.ispartofInternational Journal of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectH. perforatumen_US
dc.subjectHT29 cellsen_US
dc.subjectcolorectal canceren_US
dc.subjectapoptosisen_US
dc.subjectantimutagenic activityen_US
dc.subjectproapoptotic effecten_US
dc.subjectphytochemicalen_US
dc.titleProapoptotic Effect of Hypericum perforatum (St. John's Wort) Extract in Human Colorectal Adenocarcinoma Cell Line HT29en_US
dc.typeArticleen_US

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