Effects of glycerol additions on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectant

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.authoridAkcay, Ergun/0000-0002-7491-5671
dc.contributor.authorTekin, N.
dc.contributor.authorSecer, S.
dc.contributor.authorAkcay, E.
dc.contributor.authorBozkurt, Y.
dc.contributor.authorKayam, S.
dc.date.accessioned2024-09-18T21:05:17Z
dc.date.available2024-09-18T21:05:17Z
dc.date.issued2007
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe study was conducted to evaluate the effects of different extenders, cryoprotectants and glycerol additions on the post-thaw fertility and interactions between extenders and cryoprotectants during cryopreservation. Semen was collected by abdominal stripping from 30 adult male rainbow trout (Oncorhynchus mykiss Walbaum, 1792) and diluted with three different extenders (Erdahl-Graham, Lahnsteiner, glucose-based) containing 15% DMA, 15% DMSO, 15% DMA + 1% glycerol and 15% DMSO + 1% glycerol at a ratio of 1 : 2. Diluted samples were frozen as 0.1 ml pellets directly on dry ice (solid CO2, -79 degrees C). Eggs were pooled from 10 females. Fertilization was applied in plastic dishes and 600 eggs were used in each fertilization trial. Pellets were thawed in their own extenders (30 degrees C) at a ratio of 1 : 10. 0.3% NaCl was used for activating motility. Sperm-egg ratio was approximately 3 x 10(6) sp per egg. Experimental success was determined as the percentage of eyed-eggs 25 days after fertilization. The highest eyed-egg rate (49.3%) was obtained from semen frozen with glucose-based extender containing 15% DMSO + 1% glycerol. Our results indicate that the glucose-based extender containing DMSO is a useful combination, but that the addition of glycerol does not have a positive effect on post-thaw fertility, and that interaction of the extender-cryoprotectant is also important in the cryopreservation of rainbow trout semen.en_US
dc.identifier.doi10.1111/j.1439-0426.2006.00792.x
dc.identifier.endpage63en_US
dc.identifier.issn0175-8659
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-33846301061en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage60en_US
dc.identifier.urihttps://doi.org/10.1111/j.1439-0426.2006.00792.x
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13495
dc.identifier.volume23en_US
dc.identifier.wosWOS:000243475100010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBlackwell Publishingen_US
dc.relation.ispartofJournal of Applied Ichthyologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOncorhynchus-Mykiss Spermen_US
dc.subjectCryopreserved Spermatozoaen_US
dc.subjectEquilibration Timeen_US
dc.subjectSemenen_US
dc.subjectPreservationen_US
dc.subjectSuitabilityen_US
dc.subjectSucroseen_US
dc.subjectAbilityen_US
dc.subjectStrawsen_US
dc.titleEffects of glycerol additions on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectanten_US
dc.typeArticleen_US

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