Molecular characterization and genetic diversity analysis of mandarin genotypes by SSR and SRAP markers

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dc.authoridUZUN, Aydin/0000-0001-9496-0640
dc.contributor.authorKacar, Yildiz
dc.contributor.authorUzun, Aydin
dc.contributor.authorPolat, Ilknur
dc.contributor.authorYesiloglu, Turgut
dc.contributor.authorYilmaz, Bilge
dc.contributor.authorGulsen, Osman
dc.contributor.authorTuzcu, Onder
dc.date.accessioned2024-09-18T20:25:19Z
dc.date.available2024-09-18T20:25:19Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of the present study was to characterize the mandarin germplasm conserved at the Tuzcu Citrus Variety Collection at Cukurova University in Turkey using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) based molecular approaches. Genetic characterization of 65 mandarins was performed using 14 SSR markers and 21 SRAP markers. We originally tested 26 SSR markers, and 14 of these markers were selected due to their high polymorphism information content in the molecular assays. Thirty-eight alleles were detected at 14 loci. The number of alleles per SSR locus ranged from 2 to 4, with a mean of 2.7 alleles. The most informative loci were CAGG 9, CAT 01 and TAA 52 (4 alleles/primer). From the SRAP analysis, a total of 187 alleles were generated, and the polymorphism rate was 77%. The number of alleles detected by a single primer set ranged from 4 to 15, with an average of 8.90. The UPGMA dendrogram, defined by SSR and SRAP markers, revealed the genetic relatedness of the mandarin genotypes. These findings can be used to guide future breeding studies and germplasm management of these mandarin genotypes.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [105G072]; General Directorate of Agricultural Research of Ministry of Food and Agriculture Turkeyen_US
dc.description.sponsorshipThe authors would like to thank The Scientific and Technological Research Council of Turkey (TUBITAK; project number: 105G072) and the General Directorate of Agricultural Research of Ministry of Food and Agriculture Turkey for funding this study.en_US
dc.identifier.endpage521en_US
dc.identifier.issn1459-0255
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84874532737en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage516en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10236
dc.identifier.volume11en_US
dc.identifier.wosWOS:000315885300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWfl Publen_US
dc.relation.ispartofJournal of Food Agriculture & Environmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenetic diversityen_US
dc.subjectmandarinen_US
dc.subjectmarkersen_US
dc.subjectSSRen_US
dc.subjectSRAPen_US
dc.titleMolecular characterization and genetic diversity analysis of mandarin genotypes by SSR and SRAP markersen_US
dc.typeArticleen_US

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