Identification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkey

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dc.contributor.authorKoc, A. Nedret
dc.contributor.authorAtalay, Mustafa A.
dc.contributor.authorInci, Melek
dc.contributor.authorSariguzel, Fatma M.
dc.contributor.authorSav, Hafize
dc.date.accessioned2024-09-18T20:15:06Z
dc.date.available2024-09-18T20:15:06Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractDermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR.en_US
dc.description.sponsorshipDepartment of Scientific Research Projects (BAP) Commission, Erciyes University, Kayseri, Turkey [TSA-11-3244]en_US
dc.description.sponsorshipThis study (Number: TSA-11-3244) was supported by the Department of Scientific Research Projects (BAP) Commission, Erciyes University, Kayseri, Turkey.en_US
dc.identifier.doi10.1111/myc.12602
dc.identifier.endpage354en_US
dc.identifier.issn0933-7407
dc.identifier.issn1439-0507
dc.identifier.issue5en_US
dc.identifier.pmid28220547en_US
dc.identifier.scopus2-s2.0-85013392407en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage348en_US
dc.identifier.urihttps://doi.org/10.1111/myc.12602
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9451
dc.identifier.volume60en_US
dc.identifier.wosWOS:000399002800008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofMycosesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdermatophyte isolatesen_US
dc.subjectDNA sequencing analysisen_US
dc.subjectgenotypingen_US
dc.subjectrepetitive sequence-based PCR by DiversiLab systemen_US
dc.subjectTrichophyton mentagrophytesen_US
dc.subjectTrichophyton rubrumen_US
dc.titleIdentification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkeyen_US
dc.typeArticleen_US

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