Critical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejuni

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dc.authoridMichel, Linda/0000-0001-7957-7248
dc.authoridSahin, Orhan/0000-0003-0262-4581
dc.contributor.authorLin, J
dc.contributor.authorSahin, O
dc.contributor.authorMichel, LO
dc.contributor.authorZhang, OJ
dc.date.accessioned2024-09-18T21:05:26Z
dc.date.available2024-09-18T21:05:26Z
dc.date.issued2003
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCmeABC functions as a multidrug efflux pump contributing to the resistance of Campylobacter to a broad range of antimicrobials. In this study, we examined the role of CmeABC in bile resistance and its contribution to the adaptation of Campylobacter jejuni in the intestinal tract of the chicken, a natural host and a major reservoir for Campylobacter. Inactivation of cmeABC drastically decreased the resistance of Campylobacter to various bile salts. Addition of choleate (2 mM) in culture medium impaired the in vitro growth of the cmeABC mutants but had no effect on the growth of the wild-type strain. Bile concentration varied in the duodenum, jejunum, and cecum of chicken intestine, and the inhibitory effect of the intestinal extracts on the in vitro growth of Campylobacter was well correlated with the total bile concentration in the individual sections of chicken intestine. When inoculated into chickens, the wild-type strain colonized the birds as early as day 2 postinoculation with a density as high as 10(7) CFU/g of feces. In contrast, the cmeABC mutants failed to colonize any of the inoculated chickens throughout the study. The minimum infective dose for the cmeABC mutant was at least 2.6 x 10(4)-fold higher than that of the wild-type strain. Complementation of the cmeABC mutants with a wild-type cmeABC allelle in trans fully restored the in vitro growth in bile-containing media and the in vivo colonization to the levels of the wild-type strain. Immunoblotting analysis indicated that CmeABC is expressed and immunogenic in chickens experimentally infected with C. jejuni. Together, these findings provide compelling evidence that CmeABC, by mediating resistance to bile salts in the intestinal tract, is required for successful colonization of C. jejuni in chickens. Inhibition of CmeABC function may not only control antibiotic resistance but also prevent the in vivo colonization of pathogenic Campylobacter.en_US
dc.description.sponsorshipNIDDK NIH HHS [R01 DK063008, DK063008] Funding Source: Medlineen_US
dc.identifier.doi10.1128/IAI.71.8.4250-4259.2003
dc.identifier.endpage4259en_US
dc.identifier.issn0019-9567
dc.identifier.issn1098-5522
dc.identifier.issue8en_US
dc.identifier.pmid12874300en_US
dc.identifier.scopus2-s2.0-0042265519en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4250en_US
dc.identifier.urihttps://doi.org/10.1128/IAI.71.8.4250-4259.2003
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13569
dc.identifier.volume71en_US
dc.identifier.wosWOS:000184392700004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.ispartofInfection and Immunityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEscherichia-Colien_US
dc.subjectEpithelial-Cellsen_US
dc.subjectMechanismsen_US
dc.subjectProteinen_US
dc.subjectChicksen_US
dc.subjectIdentificationen_US
dc.subjectSequenceen_US
dc.subjectSystemen_US
dc.subjectToxinen_US
dc.subjectCampylobacter-Jejuni-81-176en_US
dc.titleCritical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejunien_US
dc.typeArticleen_US

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