Loss of heterozygosity of chromosome 13q33-34 region and molecular analysis of ING1 and p53 genes in bladder carcinoma

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dc.authoridIgci, Yusuf Ziya/0000-0001-9187-3728
dc.authoridCakmak, Ecir Ali/0000-0003-2735-2105
dc.authoridONUR, ELIF/0000-0002-1690-3170
dc.contributor.authorIgci, Mehri
dc.contributor.authorArslan, Ahmet
dc.contributor.authorErturhan, Sakip
dc.contributor.authorIgci, Yusuf Ziya
dc.contributor.authorPala, Elif
dc.contributor.authorGogebakan, Bulent
dc.contributor.authorKarakok, Metin
dc.date.accessioned2024-09-18T19:47:57Z
dc.date.available2024-09-18T19:47:57Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCancer is a consequence of accumulation of genetic and epigenetic alterations in the cell which can lead to activation of oncogenes or inactivation of tumor suppressor genes (TSG). Since members of ING family were discovered as TSGs in different cancer types, it was aimed to analyze the chromosome 13q33-34 region, ING1 and p53 genes in bladder cancer. 30 paired normal and tumor tissues were investigated in terms of microdeletion of chromosome 13q33-34 region, ING1 expression and mutation status of ING1 and p53 genes. Because there is no data available about the transcription factors which bind to ING1 promoter, the promoter sequence was analyzed via Genomatix-MatInspector and TFSEARCH softwares. Used DS markers were D13S285, D13S1315, D13S796, D13S278, D13S158, and D13S779 where loss of heterozygosity (LOH) results were as 23.3, 20, 6.7, 3.3, 6.7, and 0 %, respectively. The highest LOH scores were obtained with markers D13S285 and D13S1315 which are flanking the ING1. Seven of 30 cases showed alteration in expression (p > 0.05). However, no mutation was detected in the exons of ING1. One patient showed a two-nucleotide deletion in p53 gene. However no significant TSG activity of ING1 was observed while higher activity was reported in different cancer types. As for the LOH data 13q33-34 region may contain different candidate TSGs like COL4A1, COL4A2 and SOX1. As a result of computational promoter analysis, some factors like ABL, E2F, HIF1, SOX, P53, BPTF, NRSF, c-Rel and c-ETS were associated with the promoter region. Molecular analysis of ING1 promoter warrants further analysis.en_US
dc.description.sponsorshipGaziantep University Scientific Research Projects Governing Unit [TF.10.16]en_US
dc.description.sponsorshipThis study was supported by a research project (TF.10.16) from Gaziantep University Scientific Research Projects Governing Unit. We also would like to thank Prof. Celaletdin Camci from the Department of Oncology, University of Gaziantep, for his valuable support and contribution in several stages of the study.en_US
dc.identifier.doi10.1007/s11033-014-3794-1
dc.identifier.endpage516en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue2en_US
dc.identifier.pmid25324173en_US
dc.identifier.scopus2-s2.0-84925538960en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage507en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-014-3794-1
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7241
dc.identifier.volume42en_US
dc.identifier.wosWOS:000349006900022en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject13q33-34en_US
dc.subjectBladder canceren_US
dc.subjectING1 expressionen_US
dc.subjectLoss of heterozygosityen_US
dc.subjectMutationen_US
dc.subjectTumor suppressor genesen_US
dc.titleLoss of heterozygosity of chromosome 13q33-34 region and molecular analysis of ING1 and p53 genes in bladder carcinomaen_US
dc.typeArticleen_US

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