A plasma membrane H+ATPase gene is germination-induced in wheat embryos

dc.contributor.authorCaliskan, Mahmut
dc.contributor.authorBashiardes, Stavros
dc.contributor.authorCuming, Andrew C.
dc.date.accessioned2024-09-18T20:04:31Z
dc.date.available2024-09-18T20:04:31Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe expression pattern of a germination specific plasma membrane H+-ATPase was analyzed by RTPCR and in situ RNA hybridization methods. RT-PCR results revealed that germination specific plasma membrane H+-ATPase accumulation was detectable in all organs and tissues of germinating wheat embryos. H+-ATPase expression was not observed in dry wheat embryos and in immature wheat embryos. In situ RNA hybridization indicated that germination specific plasma membrane H+-ATPase gene expression was associated with all organs of germinating wheat tissues. The accumulation of H+-ATPase mRNA was more heavily on the cells of vascular bundles and epidermal cells of coleoptiles. Since germination specific plasma membrane H+-ATPase gene was identified as a growth related gene, interest was focused on the activity of growth regulators (GA, IAA, ABA) and stress factors, NaCl and Mannitol, on H+-ATPase gene expression. The results indicated that there were not any dramatic changes in the accumulation of germination specific plasma membrane H+-ATPase gene in any case. More rigorous analysis is necessary to evaluate the effect of growth regulators on germination specific plasma membrane H+-ATPase.en_US
dc.identifier.endpage272en_US
dc.identifier.issn1684-5315
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-75749129435en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage268en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8220
dc.identifier.volume9en_US
dc.identifier.wosWOS:000275381500002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosynthesisen_US
dc.subjectcerealsen_US
dc.subjectDNAen_US
dc.subjectenzymesen_US
dc.subjectphysiologyen_US
dc.subjectplant cell cultureen_US
dc.titleA plasma membrane H+ATPase gene is germination-induced in wheat embryosen_US
dc.typeArticleen_US

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