A plasma membrane H+ATPase gene is germination-induced in wheat embryos
dc.contributor.author | Caliskan, Mahmut | |
dc.contributor.author | Bashiardes, Stavros | |
dc.contributor.author | Cuming, Andrew C. | |
dc.date.accessioned | 2024-09-18T20:04:31Z | |
dc.date.available | 2024-09-18T20:04:31Z | |
dc.date.issued | 2010 | |
dc.department | Hatay Mustafa Kemal Üniversitesi | en_US |
dc.description.abstract | The expression pattern of a germination specific plasma membrane H+-ATPase was analyzed by RTPCR and in situ RNA hybridization methods. RT-PCR results revealed that germination specific plasma membrane H+-ATPase accumulation was detectable in all organs and tissues of germinating wheat embryos. H+-ATPase expression was not observed in dry wheat embryos and in immature wheat embryos. In situ RNA hybridization indicated that germination specific plasma membrane H+-ATPase gene expression was associated with all organs of germinating wheat tissues. The accumulation of H+-ATPase mRNA was more heavily on the cells of vascular bundles and epidermal cells of coleoptiles. Since germination specific plasma membrane H+-ATPase gene was identified as a growth related gene, interest was focused on the activity of growth regulators (GA, IAA, ABA) and stress factors, NaCl and Mannitol, on H+-ATPase gene expression. The results indicated that there were not any dramatic changes in the accumulation of germination specific plasma membrane H+-ATPase gene in any case. More rigorous analysis is necessary to evaluate the effect of growth regulators on germination specific plasma membrane H+-ATPase. | en_US |
dc.identifier.endpage | 272 | en_US |
dc.identifier.issn | 1684-5315 | |
dc.identifier.issue | 3 | en_US |
dc.identifier.scopus | 2-s2.0-75749129435 | en_US |
dc.identifier.scopusquality | N/A | en_US |
dc.identifier.startpage | 268 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12483/8220 | |
dc.identifier.volume | 9 | en_US |
dc.identifier.wos | WOS:000275381500002 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.language.iso | en | en_US |
dc.publisher | Academic Journals | en_US |
dc.relation.ispartof | African Journal of Biotechnology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Biosynthesis | en_US |
dc.subject | cereals | en_US |
dc.subject | DNA | en_US |
dc.subject | enzymes | en_US |
dc.subject | physiology | en_US |
dc.subject | plant cell culture | en_US |
dc.title | A plasma membrane H+ATPase gene is germination-induced in wheat embryos | en_US |
dc.type | Article | en_US |
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