A PCR method with internal control for detection of Brucella spp. from bovine abortion samples

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dc.authoridaltug, nuri/0000-0001-5805-0340
dc.contributor.authorSolmaz, H.
dc.contributor.authorCantekin, Z.
dc.contributor.authorAltug, N.
dc.contributor.authorIlhan, Z.
dc.contributor.authorAslan, S.
dc.contributor.authorErgun, Y.
dc.date.accessioned2024-09-18T20:06:15Z
dc.date.available2024-09-18T20:06:15Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractBrucella spp. are important infectious agents in bovine abortions worldwide. The bacteriological culture of Brucella spp. is fastidious and time consuming procedure as a classical laboratory method. Brucella spp. can be detected by using different molecular techniques. The aim of this study is to develop a PCR technique with an internal control for detection of Brucella spp. from bovine abortion samples. For this purpose, the sensitivities of three different primer pairs (BgF/BgR, B4/B5 and JP-R/JP-F) were compared. Bovine 12S gene specific primer pairs (12SM-FW/12SBT-REV2) were used as an internal control. The sensitivity of BgF/BgR primers was found higher than the other primer sets. A PCR assay was developed by combining BgF/BgR primer sets and primers for bovine 12S. This protocol was tested and validated by using abomasal contents of two Brucella-positive and eighteen Brucella-negative clinical samples. In conclusion, the developed PCR method with an internal positive control has a potential for use in direct detection and identification of the Brucella spp. from bovine abortion samples.en_US
dc.identifier.endpage257en_US
dc.identifier.issn0035-1555
dc.identifier.issn2258-0646
dc.identifier.issue9-10en_US
dc.identifier.startpage253en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8416
dc.identifier.volume165en_US
dc.identifier.wosWOS:000343280600001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherEcole Nationale Veterinaire Toulouseen_US
dc.relation.ispartofRevue De Medecine Veterinaireen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrucella spp., PCRen_US
dc.subjectInternal Controlen_US
dc.titleA PCR method with internal control for detection of Brucella spp. from bovine abortion samplesen_US
dc.typeArticleen_US

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