Myrtus communis L. essential oil reduced colony formation and altered cell differentiation markers in lung cancer spheroids

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dc.contributor.authorTerzi, Menderes Yusuf
dc.contributor.authorGulbol-Duran, Gulay
dc.date.accessioned2024-09-18T19:47:58Z
dc.date.available2024-09-18T19:47:58Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIt was aimed to investigate the effect of Myrtus communis L. (MC) essential oil on cancer spheroids derived from A549 cells by analyzing cell viability and expression levels of prominent cancer stem cell and apoptosis markers. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide assay was per-formed to analyze cytotoxicity of MC essential oil for 24-h and 72-h. A549 cells were cultured in plates with low adherence to obtain spheroids. The effect of MC essential oil was assessed on the clonogenicity of the spheroids. Quantitative reverse-transcription polymerase chain reaction method was utilized to measure relative expressions of cluster of differentiation 44 (CD44), prominin-1 (PROM1), POU class 5 homeobox 1 (POU5F1), ATP binding cassette subfamily G member-2 (ABCG2), and caspase-3 (CASP3). Relative protein levels of ABCG2 and CASP3 were also evaluated with Western blot analysis. MC essential oil reduced cell viability of A549 cells after 24-h and 72-h. A reduction was observed in number of cancer spheroids after MC essential oil treatment for 7 days. After 24h of MC essential oil treatment, PROM1, POU5F1, ABCG2, and CASP3 gene expressions decreased while returned to basal levels after 72h. CD44 levels did not change upon MC essential oil treatment. ABCG2 protein level reduced after 24-h MC essential oil treatment and returned to its basal level after 72-h. CASP3 protein levels did not change at either time points. MC essential oil exerted its anti-tumorigenic effects onto lung cancer spheroids derived from A549 cells by reducing cell viability of cancer cells, inhibiting spheroid formation efficiency, and downregulating several cancer stem cell markers.en_US
dc.description.sponsorshipHatay Mustafa Kemal University Scientific Research Projects [20.M.039]en_US
dc.description.sponsorshipThis project was supported by Hatay Mustafa Kemal University Scientific Research Projects Funding with the project number 20.M.039. Grateful thanks to Prof. Dr. Alpaslan Kaya, in Hatay Mustafa Kemal University, Faculty of Agriculture, Department of Field Crops, for providing us the MC essential oil extract.en_US
dc.identifier.doi10.1080/0972060X.2023.2234440
dc.identifier.endpage638en_US
dc.identifier.issn0972-060X
dc.identifier.issn0976-5026
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85168706423en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage626en_US
dc.identifier.urihttps://doi.org/10.1080/0972060X.2023.2234440
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7264
dc.identifier.volume26en_US
dc.identifier.wosWOS:001053427200004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal of Essential Oil Bearing Plantsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectA549 cell lineen_US
dc.subjectlung cancer spheroiden_US
dc.subjectMyrtus communis L. essential oilen_US
dc.titleMyrtus communis L. essential oil reduced colony formation and altered cell differentiation markers in lung cancer spheroidsen_US
dc.typeArticleen_US

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