Detection of slime and methicillin resistance genes in Staphylococci isolated from nasal samples of patients with orthopaedic implants

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dc.contributor.authorDuran, Nizami
dc.contributor.authorDogramaci, Yunus
dc.contributor.authorDemir, Cemil
dc.contributor.authorOzer, Burcin
dc.contributor.authorKalacı, Aydıner
dc.date.accessioned2024-09-18T20:52:58Z
dc.date.available2024-09-18T20:52:58Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: The purposes of the present study were (1) to determine the prevalence of mecA and femA genes, (2) to investigate the presence of icaA and icaD genes responsible for slime synthesis, and (3) to search in vitro slime synthesis by staphylococcal strains isolated from the nares of patients with orthopaedic implants using the Congo red agar (CRA) plate test. Material/Methods: Staphylococci strains were defined by multiplex polymerase chain reaction (PCR) technique to determine intercellular adhesion genes icaA and icaD. Slime production capability was searched by the CRA plate test, phenotypically. Also, the presence of mecA and femA genes was determined by PCR in all strains. Results: The presence of icaA and icaD was detected in 101 isolates of 134 (75.4%) strains. This ratio was 74.8% (89 of 119) among the Staphylococcus epidermidis and 80% (12 of 15) among the Staphylococcus aureus isolates. A total of 63.4% of all the strains were found to be icaA and icaD positive as well as slime-forming on the CRA plate test. The percentage of icaA-and icaD-negative strains was 36.6%, and all of them were negative on the CRA plate test. Although femA presence was detected in all 15 (11.2%) S. aureus isolates, a total of 5 (3.7%) isolates carried the mecA gene. Conclusions: The frequency of icaA and icaD genes was determined to be of high prevalence among staphylococcal isolates. The staphylococcal strains that were found in the nasal flora of patients with orthopaedic implants may be important potential sources of infection for these patients.en_US
dc.identifier.endpageBR277en_US
dc.identifier.issn1643-3750
dc.identifier.issue8en_US
dc.identifier.pmid20671608en_US
dc.identifier.scopus2-s2.0-77955486135en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpageBR271en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11513
dc.identifier.volume16en_US
dc.identifier.wosWOS:000280762400009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInt Scientific Information, Incen_US
dc.relation.ispartofMedical Science Monitoren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectorthopaedicen_US
dc.subjectimplanten_US
dc.subjectinfectionen_US
dc.subjectslimeen_US
dc.subjectstaphylococcien_US
dc.titleDetection of slime and methicillin resistance genes in Staphylococci isolated from nasal samples of patients with orthopaedic implantsen_US
dc.typeArticleen_US

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