Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2

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dc.contributor.authorYildirim, Ayse
dc.contributor.authorDuran, Gulay Gulbol
dc.contributor.authorDuran, Nizami
dc.contributor.authorJenedi, Kemal
dc.contributor.authorBolgul, Behiye Sezgin
dc.contributor.authorMiraloglu, Meral
dc.contributor.authorMuz, Mustafa
dc.date.accessioned2024-09-18T20:02:32Z
dc.date.available2024-09-18T20:02:32Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses. Material/Methods: All experiments were carried out in HEp-2 cell cultures. Proliferation assays were performed in 24-well flat bottom microplates. We inoculated 1x10(5) cells per ml and RPMI 1640 medium with 10% fetal calf serum into each well. Studies to determine cytotoxic effect were performed. To investigate the presence of antiviral activity of propolis samples, different concentrations of propolis (3200, 1600, 800, 400, 200, 100, 75, 50, and 25 mu g/mL) were added into the culture medium. The amplifications of HSV-1 and HSV-2 DNA were performed by realtime PCR method. Acyclovir (Sigma, USA) was chosen as a positive control. Cell morphology was evaluated by scanning electron microscopy (SEM). Results: The replication of HSV-1 and HSV-2 was significantly suppressed in the presence of 25, 50, and 100 mu g/mL of Hatay propolis. We found that propolis began to inhibit HSV-1 replication after 24 h of incubation and propolis activity against HSV-2 was found to start at 48 h following incubation. The activity of propolis against both HSV-1 and HSV-2 was confirmed by a significant decrease in the number of viral copies. Conclusions: We determined that Hatay propolis samples have important antiviral effects compared with acyclovir. In particular, the synergy produced by antiviral activity of propolis and acyclovir combined had a stronger effect against HSV-1 and HSV-2 than acyclovir alone.en_US
dc.identifier.doi10.12659/MSM.897282
dc.identifier.endpage430en_US
dc.identifier.issn1643-3750
dc.identifier.pmid26856414en_US
dc.identifier.scopus2-s2.0-84958038125en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage422en_US
dc.identifier.urihttps://doi.org/10.12659/MSM.897282
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7844
dc.identifier.volume22en_US
dc.identifier.wosWOS:000369911600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInt Scientific Information, Incen_US
dc.relation.ispartofMedical Science Monitoren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntiviral Agentsen_US
dc.subjectDNA Replicationen_US
dc.subjectHerpesvirus 1en_US
dc.subjectHumanen_US
dc.subjectHerpesvirus 2, Humanen_US
dc.subjectPropolisen_US
dc.titleAntiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2en_US
dc.typeArticleen_US

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