EFFECT OF DIFFERENT CRYOPROTECTANTS (GLYCEROL, METHANOL AND DIMETHYL SULFOXIDE) ON POST-THAW QUALITY, VIABILITY, FERTILIZATION ABILITY AND DNA DAMAGE OF CRYOPRESERVED NILE TILAPIA (Oreochromis niloticus) SPERMATOZOA

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.authoridYeni, Deniz/0000-0002-9105-5677
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorYeni, Deniz
dc.date.accessioned2024-09-18T20:13:34Z
dc.date.available2024-09-18T20:13:34Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBACKGROUND: Cryopreservation of sperm from different fish species requires different protocols. Therefore, it is necessary to perform studies to establish reliable procedures for each species. OBJECTIVE: Experiments were designed to analyse the effect of different types of cryoprotectants on post-thaw motility, viability and fertility as well as cryoresistance of cryopreserved Nile tilapia (Oreochromis niloticus) sperm. MATERIALS AND METHODS: Sperm samples were diluted with an ionic extender containing glycerol (Gly), methanol (MeOH) and dimethyl sulfoxide (DMSO) at ratios of 5, 10 and 15 % respectively. Diluted samples were aspirated into 0.25 ml French straws and frozen 3 cm above the surface of liquid nitrogen (LN) in a styrofoam box and stored in a LN tank. DNA damage was evaluated with the comet assay technique following cryopreservation. RESULTS: Supplementation of extender with 10% glycerol gave the highest motility rate compared with the other cryoprotectant groups (P<0.05). Differences in terms of post-thaw motility duration, cell viability and fertilization rates were not significant among treatments (P>0.05). Although Gly gave the best score (5.0 +/- 0.1%, P>0.05) at the concentration of 10%, 5% Me2SO caused significant DNA damage (15.0 +/- 1.0%, P<0.05) with the comet test. CONCLUSION: Gly or MeOH are more suitable cryoprotectants than DMSO for the cryopreservation of Nile tilapia sperm.en_US
dc.description.sponsorshipMustafa Kemal University Scientific Research Fund [MKU BAP-384]en_US
dc.description.sponsorshipThis study was funded by Mustafa Kemal University Scientific Research Fund (Project No: MKU BAP-384).en_US
dc.identifier.endpage17en_US
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue1en_US
dc.identifier.pmid30955026en_US
dc.identifier.startpage11en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9265
dc.identifier.volume40en_US
dc.identifier.wosWOS:000467311100003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCryo Lettersen_US
dc.relation.ispartofCryolettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcryodamageen_US
dc.subjectfish sperm motilityen_US
dc.subjectcryoprotectanten_US
dc.subjectDNA damageen_US
dc.subjectNile tilapiaen_US
dc.titleEFFECT OF DIFFERENT CRYOPROTECTANTS (GLYCEROL, METHANOL AND DIMETHYL SULFOXIDE) ON POST-THAW QUALITY, VIABILITY, FERTILIZATION ABILITY AND DNA DAMAGE OF CRYOPRESERVED NILE TILAPIA (Oreochromis niloticus) SPERMATOZOAen_US
dc.typeArticleen_US

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