The Effect of Erythropoietin in Preventing Ischemia-Reperfusion Injury in Ovarian Tissue Transplantation

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dc.contributor.authorKolusari, Ali
dc.contributor.authorOkyay, Ayse Guler
dc.contributor.authorKockaya, Evrim Arzu
dc.date.accessioned2024-09-18T20:19:48Z
dc.date.available2024-09-18T20:19:48Z
dc.date.issued2018
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCondensation Erythropoietin improved the survival of follicles in ovarian grafts most likely by reducing ischemic injury, by improving neoangiogenesis, and by its antioxidant effects. Objective: Ovarian tissue cryopreservation and transplantation are the only options accepted for prepubertal girls and women requiring immediate chemotherapy. Ischemia-reperfusion injury is the main obstacle for ovarian tissue transplantation. In the present study, we aimed to evaluate the effects of recombinant human erythropoietin (EPO) on tissue viability in autotransplanted rat ovaries. Study Design: Seventeen female rats were randomized into 3 groups as sham control group (n = 5), EPO-treated group (n = 6), and EPO-untreated group (n = 6). Both ovaries were excised and transplanted into a subcutaneous pouch formed at the anterior abdominal wall in the EPO-treated and untreated groups. In the EPO group, 5000 U/kg EPO was applied as local injection to the site that ovarian tissue was placed and the dose was repeated with the same route at the end of the fourth week. After 2 months, ovaries were removed and blood samples were obtained. Levels of estradiol (E-2), vascular endothelial growth factor (VEGF), VEGF-C, and lipid hydroperoxidase (LPO) and the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) were measured both in blood and tissue samples. Histopathological and morphometric analyses were also performed on tissue samples. Results: Considering serum levels, mean CAT was significantly higher (P = .003) and mean SOD (P = .033), LPO (P = .050), VEGF (P = .001), and VEGF-C (P = .024) were significantly lower in the EPO-treated group than in the untreated group. Mean serum GPX levels were similar. Significantly higher levels of E-2 were determined in the EPO group than in the untreated group. Highest serum E-2 levels were found in the sham group (P = .001). Tissue levels of GPX (1.23) and CAT (53.17) were significantly higher in the EPO group (P = .002 and P = .001, respectively). However, tissue levels of SOD and LPO, VEGF, and VEGF-C levels were significantly lower in the EPO group than those in the untreated group (P = .033, P = .050, P = .002, and P = .003, respectively). In tissue examination, the highest values of x, y axis and epithelial height were in the sham group. Mean value of the EPO group was found statistically significantly higher than that of the untreated group (P .05). In terms of antral follicle count, ordering was found as sham > EPO-treated > EPO-untreated group. Follicle counts in the EPO group were significantly higher than those in the untreated group (P 0.05). Conclusion: Erythropoietin improved the survival of follicles in ovarian grafts most likely by reducing ischemic injury, by improving neoangiogenesis, and by its antioxidant effects.en_US
dc.description.sponsorshipCoordination of Scientific Research Projects (SRP) of Yuzuncu Yil Universityen_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported financially by the Coordination of Scientific Research Projects (SRP) of Yuzuncu Yil University.en_US
dc.identifier.doi10.1177/1933719117715127
dc.identifier.endpage413en_US
dc.identifier.issn1933-7191
dc.identifier.issn1933-7205
dc.identifier.issue3en_US
dc.identifier.pmid28655290en_US
dc.identifier.scopus2-s2.0-85042316949en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage406en_US
dc.identifier.urihttps://doi.org/10.1177/1933719117715127
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9864
dc.identifier.volume25en_US
dc.identifier.wosWOS:000425172500012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Incen_US
dc.relation.ispartofReproductive Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEPOen_US
dc.subjectovarian transplantationen_US
dc.subjectantioxidanten_US
dc.subjectneoangiogenesisen_US
dc.subjecttissue viabilityen_US
dc.titleThe Effect of Erythropoietin in Preventing Ischemia-Reperfusion Injury in Ovarian Tissue Transplantationen_US
dc.typeArticleen_US

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