PCR/RFLP-based cost-effective identification of SOD2 signal (leader) sequence polymorphism (Ala-9Val) using NgoM IV: a detailed methodological approach

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dc.contributor.authorAkyol, O
dc.contributor.authorCanatan, H
dc.contributor.authorYilmaz, HR
dc.contributor.authorYuce, H
dc.contributor.authorOzyurt, H
dc.contributor.authorSogut, S
dc.contributor.authorGulec, M
dc.date.accessioned2024-09-18T20:29:44Z
dc.date.available2024-09-18T20:29:44Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Superoxide dismutases (SOD) play an important role in the protection of cells and extracellular space from the products of oxidative stress. Two allelic variants have been described for the SOD2 gene (Ile58Thr involves a C to T substitution at nucleotide residue 339 and Ala-9Val involves a T to C substitution at nucleotide residue 1183). The enzyme proteins encoded by the different alleles have been suggested to have different activity patterns. Methods: The SOD2 polymorphism was determined using a polymerase chain reaction (PCR) and RFLP techniques with restriction endonuclease NgoM IV Results: The most available results were obtained from with 20 pmol primer final concentration in PCR reaction. A total of 20 pmol seems the cost-effective primer concentration with maximum quality. There were no difference between the band quality of 1-5 units of restriction endonucleases. On the other hand, short and long incubation times seem to be similar in order to obtain sharp bands on agarose gel. Conclusions: We have extended a method of SOD2 polymorphism (Ala-9Val) in mitochondrial sequence. This method provides the ability to genotype of SOD2, and it represents a fast, reliable, cost-effective and semi-automated methodology to determine SOD2 polymorphism in order to perform large-scale population studies. (C) 2004 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.cccn.2004.03.021
dc.identifier.endpage159en_US
dc.identifier.issn0009-8981
dc.identifier.issn1873-3492
dc.identifier.issue1-2en_US
dc.identifier.pmid15193990en_US
dc.identifier.scopus2-s2.0-2942601146en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage151en_US
dc.identifier.urihttps://doi.org/10.1016/j.cccn.2004.03.021
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11034
dc.identifier.volume345en_US
dc.identifier.wosWOS:000222295200018en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofClinica Chimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmanganese superoxide dismutaseen_US
dc.subjectpolymorphismen_US
dc.subjectPCRen_US
dc.subjectsignal sequenceen_US
dc.titlePCR/RFLP-based cost-effective identification of SOD2 signal (leader) sequence polymorphism (Ala-9Val) using NgoM IV: a detailed methodological approachen_US
dc.typeArticleen_US

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