Genotypic, Antimicrobial Resistance and Virulence Profiles of Thermophilic Campylobacter Isolates in Broilers

Basit Öğe Kaydı
dc.contributor.authorAslantas, Ozkan
dc.date.accessioned2024-09-18T20:59:23Z
dc.date.available2024-09-18T20:59:23Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe objective of this study was to investigate the prevalence, antimicrobial resistance and genetic determinants of resistance of Campylobacter jejuni and Campylobacter coli isolated from commercial broiler farms in Adana and Hatay provinces, Turkey. The assessment of the genetic diversity among the isolates was determined by flaA based RFLP-Polymerase Chain Reaction (PCR) with restriction enzyme DdeI and sequence analysis of short variable regions (SVRs) of flaA and flaB-SVR genes. Antimicrobial susceptibility of the isolates was performed by disk diffusion method and tetracycline (tetO), ampicillin (blaOXA-61), aminoglycoside (aph-3-1) and multidrug efflux pump (cmeB) resistance genes were investigated by PCR as well. The genes conferring resistance to ciprofloxacin was screened by PCR and following DNA sequencing. The presence of ten virulence (flaA, virB11, racR, cadF, ciaB, dnaJ and pldA) and toxin genes (cdtA, dtB, cdtC) among the isolates was also investigated using PCR. Out of 220 cloacal swabs, 218 (99.1%) Campylobacter spp. including C. jejuni (n= 194; 89%) and C. coli (n= 24; 11%) were isolated. While all the isolates were susceptible to chloramphenicol, gentamicin and erythromycin, resistance rates for C. jejuni and C. coli isolates to nalidixic acid, ciprofloxacin, ampicillin, tetracycline and amoxicillin-clavulanic acid were determined as 86.6-100%, 86.6-100%, 45.9-45.8%, 43.3-50% and 2.6-0.0%, respectively. The tetO, blaOXA-61, cmeB and aph-3-1 genes detected in C. jejuni and C. coli isolates were 45.3-54.2%, 36.1-75%, 1.5-83.3% and 0.5-0%, respectively. The prevalence of flaA, cdtA, cdtB, cdtC, racR, cadF, ciaB, dnaJ and pldA in C. jejuni and C. coli isolates was 100-100%, 95.4-100%, 94.3-100%, 89.7-54.2%, 89.2-79.2%, 92.8-100%, 67-16.7%, 85.6-75% and 80-66.7%, respectively. virB11 gene was not detected in any of Campylobacter isolates. All flaA and flaB-SVR alleles displayed same PCR-RFLP patterns. The results of this study revealed high prevalence, pathogenic potantial, genetic diversity and antimicrobial resistance of Campylobacter spp. in broiler flocks, which highlights urgent need for implementing effective contol measures to reduce emergence and spread of antimicrobial resistant Campylobacters.en_US
dc.description.sponsorshipMustafa Kemal University Research Fund [12860]en_US
dc.description.sponsorshipThis study was supported by Mustafa Kemal University Research Fund (Project Number: 12860)en_US
dc.identifier.doi10.9775/kvfd.2016.17261
dc.identifier.endpage554en_US
dc.identifier.issn1300-6045
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85018289085en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage547en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2016.17261
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12547
dc.identifier.volume23en_US
dc.identifier.wosWOS:000401789000006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectThermophilic Campylobacteren_US
dc.subjectAntimicrobial Resistanceen_US
dc.subjectVirulenceen_US
dc.subjectGenotypingen_US
dc.titleGenotypic, Antimicrobial Resistance and Virulence Profiles of Thermophilic Campylobacter Isolates in Broilersen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin / Full Text
Boyut:
405.82 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu