The therapeutic potential and molecular mechanism of Alpha-pinene, Gamma-terpinene, and P-cymene against melanoma cells

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dc.contributor.authorAcikgul, Funda Cimen
dc.contributor.authorDuran, Nizami
dc.contributor.authorKutlu, Tuncer
dc.contributor.authorAy, Emrah
dc.contributor.authorTek, Erhan
dc.contributor.authorBayraktar, Suphi
dc.date.accessioned2024-09-18T19:52:39Z
dc.date.available2024-09-18T19:52:39Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe purpose of this study is to investigate the potential anticarcinogenic effects of three phytochemicals, namely Alpha-pinene (AP), Gamma-terpinene (GT), and P-cymene (PC), on melanoma cells (A2058 cell line). Additionally, the study aims to explore the synergistic activities of these phytochemicals with Dacarbazine, a chemotherapy drug. To understand the molecular mechanism involved in apoptosis induction in the A-2058 cell line, it was used AO/EB staining for apoptosis detection and cell cycle analysis, monitored through flow cytometry. It also determined the mRNA expression levels of different apoptosis-regulatory genes, including p53, Bax, NF-kB, Bcl-2, Bcl-xl, and caspase-3. The antitumor activities of these phytochemicals and their combinations were investigated in a subcutaneous mouse tumor model. The tumor diameter was 21.4 f 1.1 mm in the Dacarbazine treatment group and 42.4 f 3.1 mm in the control group. The antitumoral activities of AP and PC in the tumor model were similar to those of Dacarbazine. On the other hand, GT exhibited remarkable antitumoral activity, with a 1.75-fold reduction in tumor diameter compared to the Dacarbazine group. When different combinations of phytochemicals and Dacarbazine were used, the GT plus Dacarbazine treatment group was found to have a 3.5- fold reduction in tumor diameter compared to the Dacarbazine group. The tumor diameters in the Dacarbazine, AP plus GT, GT plus Dacarbazine, and AP plus Dacarbazine treatment groups were 21.4 f 1.1, 7.6 f 2.2, 8.6 f 0.5, and 6.2 f 1.9 mm, respectively.en_US
dc.description.sponsorshipScientific Research Fund of Hatay Mustafa Kemal Universityen_US
dc.description.sponsorshipFunding This study was supported by the Scientific Research Fund of Hatay Mustafa Kemal University.en_US
dc.identifier.doi10.1016/j.heliyon.2024.e36223
dc.identifier.issn2405-8440
dc.identifier.issue17en_US
dc.identifier.scopus2-s2.0-85202049833en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.heliyon.2024.e36223
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7578
dc.identifier.volume10en_US
dc.identifier.wosWOS:001303147600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCell Pressen_US
dc.relation.ispartofHeliyonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlpha-pineneen_US
dc.subjectGamma-terpineneen_US
dc.subjectP-cymeneen_US
dc.subjectAntiapoptotic geneen_US
dc.subjectProapoptotic geneen_US
dc.subjectMelanomaen_US
dc.subjectSynergistic effecten_US
dc.titleThe therapeutic potential and molecular mechanism of Alpha-pinene, Gamma-terpinene, and P-cymene against melanoma cellsen_US
dc.typeArticleen_US

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