In vitro apoptotic and antiproliferative effects of propranolol on human breast cancer cells

Basit Öğe Kaydı
dc.contributor.authorAlizade, Ares
dc.contributor.authorTerzi, Menderes Yusuf
dc.date.accessioned2024-09-18T19:54:23Z
dc.date.available2024-09-18T19:54:23Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractBreast cancer is an issue of concern with increasing incidence among women worldwide. Propranolol, as an antihypertensive drug, exerts anticancer effects too. We conducted this study to analyze the in vitro apoptotic and antiproliferative effects of propranolol in human MCF-7 breast cancer cells. MCF-7 cells were seeded into 6 -well plates and treated with 50 mu L propranolol for 24 hours. After cell homogenization, the levels of pro-apoptotic proteins BCL2 associated X (BAX), apoptosis inducing factor (AIF), C/-EBP homologous protein (GADD153), and glucose -regulated protein 78 (GRP78), anti-apoptotic protein BCL2 apoptosis regulator (BCL-2), and cycle -regulator WEE1 G2 checkpoint kinase (WEE1) were measured with ELISA. Propranolol significantly upregulated pro-apoptotic proteins AIF, BAX, GADD153, and GRP78 while downregulated anti-apoptotic protein BCL2. The level of WEE1, as the main regulatory cell cycle protein at the G2/M checkpoint, significantly increased after propranolol treatment. Propranolol inhibited the proliferation of MCF-7 human breast cancer cells by upregulating pro-apoptotic factors AIF, BAX, GADD153 and GRP78 and by downregulating antiapoptotic BCL2. Elevated WEE1 levels after propranolol treatment might lead the tumor cells into a sustained cell -cycle arrest which eventually resulted in caspase-dependent or -independent mitochondrial or endoplasmic-reticulum stress -induced apoptosis. So, propranolol can be utilized as a potential therapeutic agent in breast cancer therapy.en_US
dc.description.sponsorshipHatay Mustafa Kemal University Scientific Research Projects Funding; [18.M.105]en_US
dc.description.sponsorshipThis project was supported by Hatay Mustafa Kemal University Scientific Research Projects Funding (Project number: 18.M.105).en_US
dc.identifier.doi10.56042/ijeb.v62i05.6304
dc.identifier.endpage331en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.issue5en_US
dc.identifier.startpage326en_US
dc.identifier.urihttps://doi.org/10.56042/ijeb.v62i05.6304
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7708
dc.identifier.volume62en_US
dc.identifier.wosWOS:001222087800005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal of Experimental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnitcanceren_US
dc.subjectAntitumorigenic activityen_US
dc.subjectMCF7en_US
dc.titleIn vitro apoptotic and antiproliferative effects of propranolol on human breast cancer cellsen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
Ä°sim:
7708.pdf
Boyut:
2.31 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
Ä°ndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu