Some Anti-Inflammatory Agents Inhibit Esterase Activities of Human Carbonic Anhydrase Isoforms I and II: An In Vitro Study

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dc.authoridKilinc, Namik/0000-0002-9102-1370
dc.authoridSENGUL, BULENT/0000-0002-9998-6564
dc.contributor.authorAlim, Zuhal
dc.contributor.authorKilinc, Namik
dc.contributor.authorIsgor, Mehmet M.
dc.contributor.authorSengul, Bulent
dc.contributor.authorBeydemir, Sukru
dc.date.accessioned2024-09-18T19:54:31Z
dc.date.available2024-09-18T19:54:31Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCarbonic anhydrases (CAs) are known as a drug-target enzymes. The inhibitors of the enzyme are important compounds for discovering new therapeutic agents and understanding in detail protein-drug interactions at the molecular level. For this purpose, the invitro effects of some anti-inflammatory agents such as tenoxicam, fluorometholone acetate, and dexamethasone were investigated on esterase activity of human erythrocyte CA-I and CA-II in this study. hCA-I and hCA-II were purified by affinity chromatography with a yield of 47.25% and 87%, and a specific activity of 642.8EU/mg proteins and 5576.9EU/mg proteins, respectively. SDS-PAGE was performed to determine the purity of the enzymes. Inhibitory effects of the drugs on hCA-I and hCA-II were determined by spectrophotometric method. IC50 values for hCA-I and hCA-II were 0.198, 2.18, 11.7, 0.11, 17.5 and 14m using tenoxicam, fluorometholone acetate, and dexamethasone, respectively. For fluorometholone acetate and dexamethasone, K-i values from Lineweaver-Burk plots were obtained as 1.044 and 21.2m (noncompetitive) for hCA-I and 9.98 and 8.66m (non-competitive) for hCA-II. In conclusion, tenoxicam, fluorometholone acetate, and dexamethasone showed potent inhibitory effects on esterase activity of hCA-I and hCA-II isozymes under invitro conditions.en_US
dc.identifier.doi10.1111/cbdd.12561
dc.identifier.endpage863en_US
dc.identifier.issn1747-0277
dc.identifier.issn1747-0285
dc.identifier.issue4en_US
dc.identifier.pmid25808261en_US
dc.identifier.scopus2-s2.0-84942549553en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage857en_US
dc.identifier.urihttps://doi.org/10.1111/cbdd.12561
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7781
dc.identifier.volume86en_US
dc.identifier.wosWOS:000362163500051en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofChemical Biology & Drug Designen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectanti-inflammatory agentsen_US
dc.subjectcarbonic anhydraseen_US
dc.subjectdexamethasoneen_US
dc.subjectinhibitionen_US
dc.subjecttenoxicamen_US
dc.subjectfluorometholone acetateen_US
dc.titleSome Anti-Inflammatory Agents Inhibit Esterase Activities of Human Carbonic Anhydrase Isoforms I and II: An In Vitro Studyen_US
dc.typeArticleen_US

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