Linalool and eugenol exhibit apoptotic potential on hela and caco-2 cells through the modulation of src kinases and ADAMTS proteases while only eugenol displays anti-angiogenic features on HeLa cells

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dc.authoridKISACAM, Mehmet Ali/0000-0003-0521-9434
dc.contributor.authorKisacam, Mehmet Ali
dc.contributor.authorSakin, Fatih
dc.contributor.authorIrtegun-Kandemir, Sevgi
dc.contributor.authorPektanc-Sengul, Gulsum
dc.contributor.authorKurekci, Cemil
dc.date.accessioned2024-09-18T19:50:21Z
dc.date.available2024-09-18T19:50:21Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe cytotoxic effects of linalool and eugenol on HeLa and Caco-2 cancer cells were investigated. The expression of the Fyn, Hck, and ADAMTS genes and Bcl-2, Bax, VEGF proteins were determined through the real-time-qPCR and Western blot analysis, respectively. IC50 values of linalool on HeLa cell were 799.6, 1228, and 1239 mu g/mL after 24, 48, and 72 h, respectively, whereas those of eugenol were 388, 94.35, and 73.78 mu g/mL. IC50 values in Caco-2 cells were 887.7, 1259, and 1356 mu g/mL for linalool whereas those of eugenol were 452.7, 578.3, and 433.3 mu g/mL after 24, 48, and 72 h, respectively. As a control, Hek-293 IC50 values were 1047 and 556.7 mu g/mL for linalool and eugenol, respectively. Linalool at 1024 mu g/mL concentration increased Bax and Bcl-2 levels significantly in both cell lines (p < 0.001). However, eugenol treatment did not alter Bax levels (p > 0.05), but it decreased Bcl-2 levels significantly (p < 0.001). Additionally, 1024 mu g/mL linalool exposure increased Fyn gene expression in Caco-2 cells, which was not observed for HeLa cells. Unlike linalool, eugenol upregulated ADAMTS1 gene expression and decreased VEGF levels in HeLa cells. On the contrary, in Caco-2 cells, eugenol treatment did not affect ADAMTS1 levels, whilst linalool upregulated ADAMTS1 levels, especially at 1024 mu g/mL concentration. VEGF levels increased in Caco-2 cells following eugenol treatment. ADAMTS3 levels decreased in Caco-2 cells with exception of 1024 mu g/mL linalool treatment. These results indicated the anti-proliferative, apoptotic effects of plant-derived natural compounds. It is also revealed that eugenol might also display antiangiogenic properties on HeLa cells in addition to its apoptotic effects.en_US
dc.description.sponsorshipHatay Mustafa Kemal University Research Fund [18]en_US
dc.description.sponsorshipThis work was supported by Hatay Mustafa Kemal University Research Fund with research grants 18.A.004.en_US
dc.identifier.doi10.1007/s11756-021-00991-8
dc.identifier.endpage877en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85122650881en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage865en_US
dc.identifier.urihttps://doi.org/10.1007/s11756-021-00991-8
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7384
dc.identifier.volume77en_US
dc.identifier.wosWOS:000740429100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectADAMTSen_US
dc.subjectCellular Apoptosisen_US
dc.subjectCancer cellsen_US
dc.subjectLinaloolen_US
dc.subjectEugenolen_US
dc.subjectSrc Kinaseen_US
dc.titleLinalool and eugenol exhibit apoptotic potential on hela and caco-2 cells through the modulation of src kinases and ADAMTS proteases while only eugenol displays anti-angiogenic features on HeLa cellsen_US
dc.typeArticleen_US

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