Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey

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dc.authoridMUSETTI, Rita/0000-0001-8459-0869
dc.authoridERMACORA, Paolo/0000-0003-0757-7956
dc.contributor.authorSertkaya, G
dc.contributor.authorMartini, M
dc.contributor.authorErmacora, P
dc.contributor.authorMusetti, R
dc.contributor.authorOsler, R
dc.date.accessioned2024-09-18T20:54:07Z
dc.date.available2024-09-18T20:54:07Z
dc.date.issued2005
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractDuring the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and plum, gave positive results in PCR assays. RFLP analysis using SspI and BsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases. Stone fruit trees, including a local apricot variety ('Sakit') and a pear sample, were found to be infected with European stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma. infecting almond, apricot, myrobalan plum and peach; ESFY phytoplasma infecting Japanese plum was previously reported.en_US
dc.identifier.doi10.1007/BF02981306
dc.identifier.endpage390en_US
dc.identifier.issn0334-2123
dc.identifier.issn1876-7184
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-23644436144en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage380en_US
dc.identifier.urihttps://doi.org/10.1007/BF02981306
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11604
dc.identifier.volume33en_US
dc.identifier.wosWOS:000231194800011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPhytoparasiticaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpearen_US
dc.subjectphytoplasmaen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectrestriction fragment length polymorphismen_US
dc.subjectstone fruiten_US
dc.subjecttransmission electron microscopyen_US
dc.titleDetection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkeyen_US
dc.typeArticleen_US

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