Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from Turkish cattle

Basit Öğe Kaydı
dc.authoridgulacti, irem/0000-0002-9240-201X
dc.contributor.authorAslantas, Ö
dc.contributor.authorErdogan, S
dc.contributor.authorCantekin, Z
dc.contributor.authorGülaçti, I
dc.contributor.authorEvrendilek, GA
dc.date.accessioned2024-09-18T20:19:51Z
dc.date.available2024-09-18T20:19:51Z
dc.date.issued2006
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe objective of this study was to collect rectal swabs from the cattle in a slaughterhouse located in Hatay (Turkey) immediately after slaughter for the isolation and characterization of verotoxin-producing Escherichia coli O157 in each month during a 1-year period. The rectal swab samples were analyzed for the isolation of E. coli O157 through preenrichment, immunomagnetic separation and selective plating on CT-SMAC agar. E. coli O157 was isolated from 77 (13.6%) of the samples. The presence of E. coli O157 changed during a 1-year period, in that the occurrence of E. coli O157 was the highest in July and November and lowest in February. A total of 66 isolates out of 77 were seroytpe O157:H7 and 11 were serotype O157:NM. PCR analysis of E. coli O157 virulence genes revealed that all O157:H7/NM were positive for rbf(O157), 74 positive for EhlyA, 72 positive for eaeA, 62 positive for vtx2, and 3 positive for both vtx1 and vtx2. It was presented by cytotoxicity tests that many of E. coli O157 isolates showed high cytotoxicity on Vero cells. All of the isolates containing EhlyA showed enterohaemolysin production. (c) 2005 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.ijfoodmicro.2005.08.005
dc.identifier.endpage342en_US
dc.identifier.issn0168-1605
dc.identifier.issn1879-3460
dc.identifier.issue3en_US
dc.identifier.pmid16269196en_US
dc.identifier.scopus2-s2.0-31544465272en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage338en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijfoodmicro.2005.08.005
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9900
dc.identifier.volume106en_US
dc.identifier.wosWOS:000235570300014en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Food Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEscherichia coli O157en_US
dc.subjectTurkeyen_US
dc.subjectcattleen_US
dc.subjectIMSen_US
dc.subjectPCRen_US
dc.titleIsolation and characterization of verocytotoxin-producing Escherichia coli O157 from Turkish cattleen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ N/A ]
İsim:
Tam Metin / Full Text
Boyut:
108.06 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu