Bimolecular fluorescence complementation assay to explore protein- protein interactions of the Yersinia virulence factor YopM

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dc.authoridEvran, Serap/0000-0001-6676-4888
dc.contributor.authorUgurlu, Ozge
dc.contributor.authorEvran, Serap
dc.date.accessioned2024-09-18T20:55:30Z
dc.date.available2024-09-18T20:55:30Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractYersinia outer protein M (YopM) is one of the effector proteins and essential for virulence. YopM is delivered by the Yersinia type III secretion system (T3SS) into the host cell, where it shows immunosuppressive effect through interaction with host proteins. Therefore, protein-protein interactions of YopM is significant to understand its molecular mechanism. In this study, we aimed to explore protein protein interactions of YopM with the two components of T3SS, namely LcrV and LcrG. We used bimolecular fluorescence complementation (BiFC) assay and monitored the reassembly of green fluorescence protein in Escherichia coli. As an indicator of the protein-protein interaction, we monitored the in vivo reconstitution of fluorescence by measuring fluorescence intensity and imaging the cells under fluorescence microscope. We showed, for the first time, that YopM interacts with LcrG, but not with LcrV. Here, we propose BiFC assay as a simple method to screen novel interaction partners of YopM. (c) 2021 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.bbrc.2021.10.039
dc.identifier.endpage48en_US
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.pmid34689104en_US
dc.identifier.scopus2-s2.0-85117399040en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage43en_US
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2021.10.039
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11872
dc.identifier.volume582en_US
dc.identifier.wosWOS:000711418700007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProtein interactionen_US
dc.subjectFluorescenceen_US
dc.subjectGreen fluorescent proteinen_US
dc.subjectProtein complementationen_US
dc.subjectEffector proteinen_US
dc.titleBimolecular fluorescence complementation assay to explore protein- protein interactions of the Yersinia virulence factor YopMen_US
dc.typeArticleen_US

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